PMID: 12480575Dec 14, 2002

Application of Agilent 2100 Bioanalyzer in the study of differential gene expression

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Cui-Hua LiuWen-Ling Zheng

Abstract

To study the application of Agilent 2100 Bioanalyzer in the study of gene differential expression. The total RNAs were extracted and purified from Saccharomyces cerevisiae to synthesize double-stranded cDNAs by reverse transcription. Restriction display-PCR was employed to obtain the cDNA fragments, which were examined by Agilent 2100 Bioanalyzer and agarose gel electrophoresis. The analysis showed that Agilent 2100 Bioanalyzer was more sensitive and faster to isolate and display the differentially expressed genes, providing at the same time accurate quantitative information for each fragment in the DNA sample. Agilent 2100 Bioanalyzer can be instrumental for the study of differential gene expression.

Related Concepts

Heat shock proteins
Electrophoresis, Agar Gel
Gene Expression
Cdna Microarrays
Saccharomyces cerevisiae
cDNA Probes
MRNA Differential Display
Nested Polymerase Chain Reaction

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