PMID: 12651231Mar 26, 2003

Application of Agilent 2100 Bioanalyzer in detection of human papilloma virus

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Cui-hua LiuWen-ling Zheng

Abstract

To explore the feasibility of using Agilent 2100 bioanalyzer in the detection and genotyping of human papilloma virus (HPV). The consensus sequence of highly conserved region (L1) and genotype-specific gene fragments of all HPV genotypes (including HPV6, 11, 16 and 18) were amplified by PCR technique using general and type-specific primers respectively. Agilent 2100 Bioanalyzer and routine agarose gel electrophoresis were then employed respectively to examine the PCR products, and the accuracy, reproducibility and sensitivity of these 2 detection techniques were compared. Agilent 2100 Bioanalyzer showed better accuracy in determining the length of the gene fragments than agarose gel electrophoresis, the accuracy of the former reaching above 95% while the latter was only 85%. Bioanalyzer was also 100 times more sensitive than agarose electrophoresis. Agilent 2100 Bioanalyzer combined with PCR is specific, accurate and sensitive for HPV detection and genotyping.

Related Concepts

Electrophoresis, Agar Gel
In Vitro Diagnostic Devices
Nested Polymerase Chain Reaction
Papillomaviridae

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