Application of alpha-aminoisobutyric acid, L-methionine, thymidine and 2-fluoro-2-deoxy-D-glucose to monitor effects of chemotherapy in a human colon carcinoma cell line

European Journal of Nuclear Medicine
H SchaiderG van Kaick

Abstract

Up to 4 h after treatment of human SW 707 colon carcinoma cells with the antineoplastic drug 4-amino-N-(2'-aminophenyl)-benzamide (GOE 1734, dinaline), the effects of tumour cell metabolism and proliferation were examined in vitro. Four tracers which can be labelled with isotopes suitable for positron emission tomography (PET) were used for this purpose: alpha-aminoisobutyric acid (AIB) and methionine to study changes in amino acid transport and protein synthesis, thymidine to observe changes in tumour proliferation and 2-fluoro-2-deoxy-D-glucose (FDG) to estimate glucose metabolism. Dinaline showed an inhibition of the sodium-dependent and -independent uptake of AIB. The methionine uptake was found to increase shortly after therapy. Thymidine incorporation into DNA was impaired and the FDG uptake showed a maximally 2.2-fold enhancement. Inhibition of AIB uptake suggests changes in amino acid transport, whereas increased uptake of methionine and FDG points to an enhancement of protein synthesis and glycolysis caused by repair mechanisms. The cytostatic and antiproliferative effect of dinaline, observed in cell growth curves, could be demonstrated by the impaired thymidine incorporation into DNA. This study demonstrates that in ...Continue Reading

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Citations

Feb 26, 2003·European Journal of Nuclear Medicine and Molecular Imaging·Karoline SpaepenChristine De Wolf-Peeters
Apr 9, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Sebastian A MüllerReingard Senekowitsch-Schmidtke
Mar 15, 2016·Frontiers in Oncology·Amarnath Challapalli, Eric O Aboagye
May 18, 2011·Oncogene·U HaberkornA Altmann
Jul 11, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Wolfgang A Weber
Jul 28, 2011·Medicinal Research Reviews·Jonathan McConathyMark M Goodman
Mar 3, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hinrich A WiederWolfgang A Weber
Mar 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hong ZhangHirohiko Tsujii

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