Application of an enzyme-linked immunosorbent-inhibition assay to quantitate the release of KS peptides into fluids of the rat subcutaneous air-pouch model and the effects of chondroprotective drugs on the release process.

Rheumatology International
P KongtawelertP Ghosh

Abstract

The effects of the chondroprotective agents (Arteparon, SP-54 and DH40J) on the release of proteoglycan degradation products (as keratan sulphate peptide fragments) from articular cartilage implanted into rat subcutaneous air pouches have been investigated by using an enzyme-linked immunosorbent-inhibition assay (ELISIA). The ELISIA technique was capable of quantitating the keratan sulphate peptides (KS peptides) in fluids within the range of 100-2,000 ng/ml by using the monoclonal antibody line 1/20/5-D-4 and human articular cartilage KS peptides as standard reagents. It was found that the levels of KS peptides present in the air-pouch fluid of rats treated with the chondroprotective drugs was significantly less than in fluid aspirated from the pouches of non-drug-treated control animals. On the basis of these findings we suggest that the assessment of KS peptide by ELISIAs may provide a useful means of monitoring proteoglycan breakdown products in biological fluids (e.g. synovial fluids or blood) and for evaluating the effects that antiarthritic drugs may have on this process.

References

Apr 1, 1979·The Biochemical Journal·J Wieslander, D Heinegård
Feb 1, 1976·The Journal of Bone and Joint Surgery. British Volume·C A McDevitt, H Muir
May 28, 1976·Biochimica Et Biophysica Acta·G E KempsonP H Horsfield
Dec 1, 1985·Arthritis and Rheumatism·E J ThonarK E Kuettner
Feb 1, 1987·British Journal of Pharmacology·P M BrooksM J Forrest
Mar 15, 1986·The Biochemical Journal·T C LaurentB Smedsrød
Jan 1, 1987·The International Journal of Biochemistry·H C KirchA M Gressner
Aug 1, 1987·Seminars in Arthritis and Rheumatism·M S Saag, J C Bennett
Aug 1, 1987·Seminars in Arthritis and Rheumatism·T Gordon, D Isenberg
May 15, 1985·The Biochemical Journal·F BonnetT E Hardingham
Nov 15, 1985·Analytical Biochemistry·D HeinegårdB Larsson
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen

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Citations

Jun 21, 2007·Analytical and Bioanalytical Chemistry·Supaporn Kradtap HartwellKate Grudpan
Aug 30, 2008·Journal of Clinical Periodontology·Sakornrat KhongkhunthianPrachya Kongtawelert

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