Application of an LC-MS/MS method for the simultaneous quantification of human intestinal transporter proteins absolute abundance using a QconCAT technique

Journal of Pharmaceutical and Biomedical Analysis
M D HarwoodA Rostami-Hodjegan

Abstract

Transporter proteins expressed in the gastrointestinal tract play a major role in the oral absorption of some drugs, and their involvement may lead to drug-drug interaction (DDI) susceptibility when given in combination with drugs known to inhibit gut wall transporters. Anticipating such liabilities and predicting the magnitude of the impact of transporter proteins on oral drug absorption and DDIs requires quantification of their expression in human intestine, and linking these to data obtained through in vitro experiments. A quantitative targeted absolute proteomic method employing liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) together with a quantitative concatenation (QconCAT) strategy to provide proteotypic peptide standards has been applied to quantify ATP1A1 (sodium/potassium-ATPase; Na/K-ATPase), CDH17 (human peptide transporter 1; HPT1), ABCB1 (P-glycoprotein; P-gp), ABCG2 (breast cancer resistance protein; BCRP), ABCC2 (multidrug resistance-associated protein 2; MRP2) and SLC51A (Organic Solute Transporter subunit alpha; OST-α), in human distal jejunum (n=3) and distal ileum (n=1) enterocyte membranes. Previously developed selected reaction monitoring (SRM) schedules were optimised to enable quan...Continue Reading

References

Jul 20, 1999·The Journal of Clinical Investigation·B GreinerH K Kroemer
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nazzareno BallatoriNa Li
Feb 22, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hannah M JonesKatherine S Fenner
Aug 27, 2013·Journal of Pharmaceutical and Biomedical Analysis·C GröerS Oswald

❮ Previous
Next ❯

Citations

Mar 10, 2016·Journal of Pharmaceutical Sciences·Matthew D HarwoodGeoffrey Warhurst
Dec 16, 2016·Biopharmaceutics & Drug Disposition·Jean-Marie NicolasAnna-Lena Ungell
May 12, 2019·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Matthew HarwoodSibylle Neuhoff
Apr 5, 2017·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Brahim AchourJill Barber
May 10, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shinji YamazakiManthena V Varma
Feb 6, 2017·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Stefan F C VaessenEvita van de Steeg
Jan 22, 2020·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Narciso CoutoGeoffrey Warhurst
Feb 23, 2020·Annual Review of Analytical Chemistry·Brahim AchourJill Barber
May 1, 2020·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Yasmine ElmorsiBrahim Achour
Oct 3, 2020·Bioanalysis·Christian CaporuscioPetia Shipkova
Dec 11, 2019·Expert Opinion on Drug Metabolism & Toxicology·Teruo MurakamiNicholas Bodor
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiapeng Li, Hao-Jie Zhu
Oct 29, 2020·Clinical Pharmacology and Therapeutics·Zubida M Al-MajdoubAmin Rostami-Hodjegan
Mar 12, 2021·Critical Reviews in Analytical Chemistry·Mustafa ModiwalaPinaki Sengupta
Sep 19, 2017·Molecular Pharmaceutics·Noora SjöstedtHeidi Kidron
Sep 8, 2021·Analytical Methods : Advancing Methods and Applications·Huanhuan LiPeng Yu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Expert Opinion on Drug Metabolism & Toxicology
Teruo Murakami, Mikihisa Takano
Current Opinion in Pharmacology
Shingen MisakaMartin F Fromm
© 2021 Meta ULC. All rights reserved