Application of analytical and preparative high-speed counter-current chromatography for the separation of Z-ligustilide from a crude extract of Angelica sinensis

Phytochemical Analysis : PCA
Xiao WangChuange Cheng

Abstract

Z-Ligustilide was separated and purified from the traditional Chinese medicinal plant Angelica sinensis by high-speed counter-current chromatography (HSCCC). Analytical HSCCC was first used for the systematic selection of the two-phase solvent system. Preparative HSCCC separation was performed with a two-phase solvent system composed of petroleum ether (60-90 degrees C)-ethanol-water at an optimum volume ratio of 10:17:10 (v/v). A total of 38 mg Z-ligustilide at 98.8% purity was obtained in one step from 200 mg crude extract as determined by HPLC analysis. The structure of the target compound was identified by electron impact ionisation mass spectrometry.

References

Sep 17, 2002·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Chumpon WilasrusmeeDilip S Kittur
Dec 1, 2005·Phytochemical Analysis : PCA·Nelson FrighettoAntonio Carlos Siani
Jun 30, 2006·European Journal of Pharmacology·Qun LuHong Yang

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Citations

Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Sep 6, 2008·Phytochemical Analysis : PCA
Jul 30, 2020·Food Science & Nutrition·Wen DuanYizhuang Tang

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