Application of ATP-based bioluminescence for bioaerosol quantification: effect of sampling method

Journal of Aerosol Science
Taewon HanGediminas Mainelis

Abstract

An adenosine triphosphate (ATP)-based bioluminescence has potential to offer a quick and affordable method for quantifying bioaerosol samples. Here we report on our investigation into how different bioaerosol aerosolization parameters and sampling methods affect bioluminescence output per bacterium, and implications of that effect for bioaerosol research. Bacillus atrophaeus and Pseudomonas fluorescens bacteria were aerosolized by using a Collison nebulizer (BGI Inc., Waltham, MA) with a glass or polycarbonate jar and then collected for 15 and 60 min with: (1) Button Aerosol Sampler (SKC Inc., Eighty Four, PA) with polycarbonate, PTFE, and cellulose nitrate filters, (2) BioSampler (SKC Inc.) with 5 and 20 mL of collection liquid, and (3) our newly developed Electrostatic Precipitator with Superhydrophobic Surface (EPSS). For all aerosolization and sampling parameters we compared the ATP bioluminescence output per bacterium relative to that before aerosolization and sampling. In addition, we also determined the ATP reagent storage and preparation conditions that that do not affect the bioluminescence signal intensity. Our results show that aerosolization by a Collison nebulizer with a polycarbonate jar yields higher bioluminesce...Continue Reading

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Citations

Oct 1, 2016·Sensors·Poornachandra Papireddy VinayakaMichael J Vellekoop
Jul 2, 2017·Applied and Environmental Microbiology·Allen E Haddrell, Richard J Thomas
Mar 19, 2021·Chemical Society Reviews·Aisha J Syed, James C Anderson

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