Application of Bld-1-Embedded Elastin-Like Polypeptides in Tumor Targeting

Scientific Reports
Vijaya SarangthemRang-Woon Park

Abstract

Expression of various molecules on the surface of cancer cells compared to normal cells creates a platform for the generation of various drug vehicles for targeted therapy. Multiple interactions between ligands and their receptors mediated by targeting peptide-modified polymer could enable simultaneous delivery of a drug selectively to target tumor cells, thus limiting side effects resulting from non-specific drug delivery. In this study, we synthesized a novel tumor targeting system by using two key elements: (1) Bld-1 peptide (SNRDARRC), a recently reported bladder tumor targeting peptide identified by using a phage-displayed peptide library, and (2) ELP, a thermally responsive polypeptide. B5V60 containing five Bld-1 peptides and non-targeted ELP77 with a thermal phase-transition over 37 °C were analyzed to determine their bioactivities. Further studies confirmed the superior binding ability of B5V60 to bladder tumor cells and the cellular accumulation of B5V60 in cancer cells was dependent on the expression level of sialyl-Tn antigen (STn), a tumor-associated carbohydrate antigen. Additionally, B5V60 displayed excellent localization in bladder tumor xenograft mice after intravenous injection and was strictly confined to sia...Continue Reading

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Citations

Aug 30, 2020·International Journal of Molecular Sciences·Changrim LeeJ Andrew MacKay
Jul 12, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Aurélie Le PageTamas Fulop
Jun 12, 2018·Bioconjugate Chemistry·Maureen R Newman, Danielle S W Benoit

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Methods Mentioned

BETA
phage display
Circular Dichroism
flow cytometry
flow
confocal microscopy
Assay
infrared fluorescence
xenograft
Fluorescence
Fluorescence Imaging

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