Application of Bone Marrow-Derived Mesenchymal Stem Cells for Muscle Healing After Contusion Injury in Mice

The American Journal of Sports Medicine
Chih-Hao ChiuYi-Sheng Chan

Abstract

Skeletal muscle injuries are very common in sports medicine. Conventional therapies have limited clinical efficacy. New treatment methods should be developed to allow athletes to return to play with better function. To evaluate the in vitro differentiation potential of bone marrow-derived mesenchymal stem cells and the in vivo histologic and physiologic effects of mesenchymal stem cell therapy on muscle healing after contusion injury. Controlled laboratory study. Bone marrow cells were flushed from both femurs of 5-week-old C57BL/6 mice to establish immortalized mesenchymal stem cell lines. A total of 36 mice aged 8 to 10 weeks were used to develop a muscle contusion model and were divided into 6 groups (6 mice/group) on the basis of the different dosages of IM2 cells to be injected (0, 1.25 × 105, and 2.5 × 105 cells with/without F-127 in 100 μL of phosphate-buffered saline). Histological analysis of muscle regeneration was performed, and the fast-twitch and tetanus strength of the muscle contractions was measured 28 days after muscle contusion injury, after injections of different doses of mesenchymal stem cells with or without the F-127 scaffold beginning 14 days after contusion injury. The mesenchymal stem cell-treated musc...Continue Reading

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Citations

Oct 1, 2020·The American Journal of Sports Medicine·Chih-Hao Chiu, Yi-Sheng Chan
Oct 1, 2020·The American Journal of Sports Medicine·Iain R MurrayGeoffrey D Abrams
Jun 8, 2021·Frontiers in Bioengineering and Biotechnology·Martina SandonàValentina Saccone

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Methods Mentioned

BETA
light microscopy
flow cytometry
Fluorescence

Software Mentioned

COR Pearl Impulse
Pearl Impulse
LI

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