Application of capillary electrophoresis/ electrospray ionization-mass spectrometry to subcellular proteomics of Escherichia coli ribosomal proteins

Electrophoresis
Mehdi Moini, Hsiaoling Huang

Abstract

We introduce capillary electrophoresis-mass spectrometry (CE-MS) as an efficient means for the on-line separation and identification of protein mixtures. It was found that while CE/electrospray ionization (ESI)-MS analysis of whole-cell lysate was too complicated for the one-dimensional CE-MS analysis, the technique was useful for the analysis of protein mixtures of moderate complexity (approximately 50 intact proteins). CE/ESI-MS was applied to the subcellular proteomics of ribosomal Escherichia coli. 55 out of the 56 ribosomal proteins were detected with ease by using only approximately 3.4 ng of ribosomal proteins. In addition, it was found that the mass accuracy of the conventional MS (such as quadrupole ion traps) was good enough to identify many post-translational modifications of the intact proteins by simply comparing their measured average molecular weight with the average molecular weight predicted from gene banks.

Citations

Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Arnold GanserEva M Weissinger
Jan 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Maria Rowena N Monton, Shigeru Terabe
Feb 10, 2007·Expert Review of Proteomics·Ildikó KustosFerenc Kilár
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Jun 15, 2007·Journal of Chromatography. a·Rob HaselbergGovert W Somsen
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring
Jun 21, 2005·Electrophoresis·Danilo FliserHarald Mischak
Mar 15, 2005·Electrophoresis·David C Simpson, Richard D Smith
Mar 19, 2005·Electrophoresis·Philippe Schmitt-Kopplin, Matthias Englmann
Apr 12, 2007·Electrophoresis·Maike SniehottaHarald Mischak
Nov 25, 2005·Electrophoresis·Vladislav Dolník
Apr 11, 2009·Journal of Separation Science·Bryan R Fonslow, John R Yates
Aug 24, 2006·Electrophoresis·Yu-Fen HuangHuan-Tsung Chang
Jul 1, 2007·Proteomics. Clinical Applications·Moritz FrommbergerEric Schiffer
Feb 22, 2011·Rapid Communications in Mass Spectrometry : RCM·Mehdi MoiniSamir Bootwala
Feb 22, 2014·Biochemical and Biophysical Research Communications·Adam D CathermanNeil L Kelleher
Jan 31, 2008·Analytical Chemistry·Maureen K BungerJames L Stephenson
Oct 14, 2006·Analytical Chemistry·Selynda Garza, Mehdi Moini
Jun 15, 2006·Analytical Chemistry·James KralyNorman J Dovichi

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