Application of Circular Dichroism and Fluorescence Spectroscopies To Assess Photostability of Water-Soluble Porcine Lens Proteins

ACS Omega
Claudia HonischPaolo Ruzza

Abstract

The eye lens is mainly composed of the highly ordered water-soluble (WS) proteins named crystallins. The aggregation and insolubilization of these proteins lead to progressive lens opacification until cataract onset. Although this is a well-known disease, the mechanism of eye lens protein aggregation is not well understood; however, one of the recognized causes of proteins modification is related to the exposure to UV light. For this reason, the spectroscopic properties of WS lens proteins and their stability to UV irradiation have been evaluated by different biophysical methods including synchrotron radiation circular dichroism, fluorescence, and circular dichroism spectroscopies. Moreover, dynamic light scattering, gel electrophoresis, transmission electron microscopy, and protein digestion followed by tandem LC-MS/MS analysis were used to study the morphological and structural changes in protein aggregates induced by exposure to UV light. Our results clearly indicated that the exposure to UV radiation modified the protein conformation, inducing a loss of ordered structure and aggregation. Furthermore, we confirmed that these changes were attributable to the generation of reactive oxygen species due to the irradiation of the ...Continue Reading

References

Apr 1, 1993·Protein Engineering·E G Hutchinson, J M Thornton
Apr 29, 1998·American Journal of Human Genetics·J F Hejtmancik
Jan 11, 2000·Eye·C Slingsby, N J Clout
Apr 28, 2001·Biophysical Journal·J T Vivian, P R Callis
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Franz Narberhaus
Nov 15, 2003·The Journal of Biological Chemistry·Sarah MeehanCait E MacPhee
Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Sep 1, 2004·Experimental Eye Research·J F HejtmancikY V Sergeev
Sep 29, 2004·Journal of Molecular Biology·P EvansC Slingsby
Apr 28, 2005·The FEBS Journal·Graeme WistowThomas Vihtelic
Apr 29, 2009·International Journal of Molecular Sciences·Kira A MarkossianBoris I Kurganov
Jun 23, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Andrea HaweWim Jiskoot
Nov 26, 2010·Experimental Eye Research·Konstantin O MuranovBoris I Kurganov
Mar 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Steven BassnettGijs F J M Vrensen
Aug 23, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·David I PattisonMichael J Davies
Feb 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sean D MoranMartin T Zanni
Apr 3, 2012·Progress in Retinal and Eye Research·Barbara K Pierscionek, Justyn W Regini
May 21, 2013·Photochemistry and Photobiology·Nathaniel Schafheimer, Jonathan King
Aug 13, 2013·PloS One·Venkata Pulla Rao VendraDorairajan Balasubramanian
Aug 21, 2013·Biochemistry·Sean D MoranMartin T Zanni
Mar 7, 2014·Progress in Biophysics and Molecular Biology·Amita MishraYogendra Sharma
May 20, 2014·Progress in Biophysics and Molecular Biology·Eugene Serebryany, Jonathan A King
Feb 28, 2015·Journal of Synchrotron Radiation·Rohanah HussainGiuliano Siligardi
Mar 25, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Daria Brisker-Klaiman, Andreas Dreuw
Jun 27, 2015·Progress in Retinal and Eye Research·Peter A Campochiaro
Jun 28, 2015·Biochimica Et Biophysica Acta·Martin HaslbeckSevil Weinkauf
Feb 16, 2016·Chemical Reviews·Maan HayyanInas M AlNashef
May 3, 2016·Biochimica Et Biophysica Acta·Paolo RuzzaGiuliano Siligardi
Jan 1, 2015·Nature Reviews. Disease Primers·Dennis LamDavid F Chang
Sep 25, 2016·Food Chemistry·Mattia Di GasperoAndrea Curioni
Mar 13, 2017·Biochimie·Susmitnarayan ChaudhurySwagata Dasgupta
Apr 27, 2017·Investigative Ophthalmology & Visual Science·Kyle W RoskampRachel W Martin
Mar 11, 2018·Biochimica Et Biophysica Acta. General Subjects·Paolo RuzzaGiuliano Siligardi
Aug 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rohanah HussainGiuliano Siligardi

❮ Previous
Next ❯

Citations

Apr 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mattia Di GasperoSimone Vincenzi
Oct 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·María Florencia PignataroVerónica Isabel Dodero
May 23, 2021·International Journal of Pharmaceutics·Wolfgang KaiserPatrick Garidel

❮ Previous
Next ❯

Methods Mentioned

BETA
circular dichroism
dynamic light scattering
electrophoresis
transmission electron microscopy

Software Mentioned

CDApps
Percolator
FL
Nano
WinLab
Mascot Search Engine
Proteome Discoverer
Jasco Spectra Manager
OriginPro

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.