In the first wave of synthetic biology, genetic elements, combined into simple circuits, are used to control individual cellular functions. In the second wave of synthetic biology, the simple circuits, combined into complex circuits, form systems-level functions. However, efforts to construct complex circuits are often impeded by our limited knowledge of the optimal combination of individual circuits. For example, a fundamental question in most metabolic engineering projects is the optimal level of enzymes for maximizing the output. To address this point, combinatorial optimization approaches have been established, allowing automatic optimization without prior knowledge of the best combination of expression levels of individual genes. This review focuses on current combinatorial optimization methods and emerging technologies facilitating their applications.
Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production
Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae
Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors
A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells
Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
Characterization of 582 natural and synthetic terminators and quantification of their design constraints
Multiplex iterative plasmid engineering for combinatorial optimization of metabolic pathways and diversification of protein coding sequences
Optimal cofactor swapping can increase the theoretical yield for chemical production in Escherichia coli and Saccharomyces cerevisiae
Use of pantothenate as a metabolic switch increases the genetic stability of farnesene producing Saccharomyces cerevisiae
Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae
Biosynthesis of Taxadiene in Saccharomyces cerevisiae : selection of geranylgeranyl diphosphate synthase directed by a computer-aided docking strategy
A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories
Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae
Autonomous production of 1,4-butanediol via a de novo biosynthesis pathway in engineered Escherichia coli
Production of β-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae
CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology
Repositioning microbial biotechnology against COVID-19: the case of microbial production of flavonoids.
SynPharm and the guide to pharmacology database: A toolset for conferring drug control on engineered proteins.
Plant synthetic biology for producing potent phyto-antimicrobials to combat antimicrobial resistance.
Mitigation of host cell mutations and regime shift during microbial fermentation: a perspective from flux memory
Unraveling the roles of plant specialized metabolites: using synthetic biology to design molecular biosensors.
Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.