Application of combined liquid chromatography/mass spectrometry (LC/MS): analysis of petroporphyrins and meibomian gland waxes

Journal of Chromatographic Science
W H McFaddenN Nicolaides

Abstract

Application of the moving belt LC/MS interface is illustrated by two diverse examples, one from the field of petroporphyrin chemistry, the other, a sample of high molecular weight waxes from the steer meibomian gland (eye). Petroporphyrins are difficult of convert to derivatives suitable for GC analysis and the advantages of analysis by a combined separation/identification method are well demonstrated by the LC/MS runs. High molecular weight waxes can be saponified and derivatized for other analyses such as GC and GC/MS, but information regarding the distribution of alcohol and acid in the combined state is destroyed. This study shows that such information can be obtained by a combined LC/MS analysis without chemical manipulation of the sample.

Citations

Jun 19, 2013·Experimental Eye Research·Igor A Butovich
Aug 25, 2012·Investigative Ophthalmology & Visual Science·Igor A ButovichJ Corinna Eule
Aug 8, 2009·Progress in Retinal and Eye Research·Igor A Butovich
Mar 10, 2011·Mass Spectrometry Reviews·Achille CappielloHelga Trufelli
Jun 9, 2018·Journal of the American Society for Mass Spectrometry·Ethan M McBride, Guido F Verbeck

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