Jul 28, 2016

Application of CRISPR-Cas9 genome editing for constructing animal models of human diseases

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
Zhanhui Ou, Xiaofang Sun

Abstract

The CRISPR-Cas9 system is a new targeted nuclease for genome editing, which can directly introduce modifications at the targeted genomic locus. The system utilizes a short single guide RNA (sgRNA) to direct the endonuclease Cas9 in the genome. Upon targeting, Cas9 can generate DNA double-strand breaks (DSBs). As such DSBs are repaired by non-homologous end joining (NHEJ) or homology directed repair (HDR), therefore facilitates introduction of random or specific mutations, repair of endogenous mutations, or insertion of DNA elements. The system has been successfully used to generate gene targeted cell lines including those of human, animals and plants. This article reviews recent advances made in this rapidly evolving technique for the generation of animal models for human diseases.

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Mentioned in this Paper

Non-Homologous DNA End-Joining
Genome
Genes
Endonuclease
Graft-vs-Host Disease
RNA Editing
Genomics
Nuclease
Clustered Regularly Interspaced Short Palindromic Repeats
Cas9 endonuclease Streptococcus pyogenes

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