Application of dye-ligant chromatography to the isolation of alpha-1-proteinase inhibitor and alpha-1-acid glycoprotein

Journal of Chromatography
G Birkenmeier, G Kopperschläger

Abstract

Various Cibacron blue F3G-A substituted Sephadex G-100 gels which differ in the density of the bound dye were investigated for their applicability in the affinity chromatography of human serum proteins. Protein adsorption was found to depend strongly on the density of the covalently attached dye and on the pH of the applied buffer. A high degree of dye substitution of the gel caused binding of most of the serum proteins. Only a small number of proteins were found to appear in the breakthrough fraction. On this basis a simple and relatively mild procedure for the isolation of homogeneous alpha-1-proteinase inhibitor and alpha-1-acid glycoprotein from human serum was developed. Isolation of alpha-1-acid glycoprotein succeeded in only a single step, whereas that of alpha-1-proteinase inhibitor required two additional steps: a prior ammonium sulphate precipitation and a chromatographic step on DEAE-cellulose at pH 6.5.

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Citations

Oct 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K HuseG Kopperschläger
Jan 1, 1985·The International Journal of Biochemistry·S D Pearson, J C Gan
May 1, 1988·Journal of Biochemical and Biophysical Methods·L MiribelP Arnaud
Nov 1, 1991·Journal of Biotechnology·G Birkenmeier, G Kopperschläger
Jul 27, 2011·Journal of Proteomics·Francesco Di GirolamoMaxey C M Chung
Sep 14, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·S OláhM Boldizsár
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·T BurnoufM Radosevich
Nov 7, 1998·Preparative Biochemistry & Biotechnology·R ChaturvediP U Sarma
Dec 12, 2012·Biomarkers in Medicine·Francesco Di GirolamoLorenza Putignani
Jun 11, 2009·Scandinavian Journal of Immunology·L LevanderP Påhlsson
Mar 1, 1986·Biological Chemistry Hoppe-Seyler·O AbeH Kaya
Jul 29, 1983·Journal of Chromatography·G BirkenmeierG Kopperschläger

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