Application of ethyl esters and d3-methyl esters as internal standards for the gas chromatographic quantification of transesterified fatty acid methyl esters in food

Journal of Agricultural and Food Chemistry
Saskia Thurnhofer, Walter Vetter

Abstract

Ethyl esters (FAEE) and trideuterium-labeled methyl esters (d3-FAME) of fatty acids were prepared and investigated regarding their suitability as internal standards (IS) for the determination of fatty acids as methyl esters (FAME). On CP-Sil 88, ethyl esters of odd-numbered fatty acids eluted approximately 0.5 min after the respective FAME, and only coelutions with minor FAME were observed. Depending on the problem, one or even many FAEE can be added as IS for the quantification of FAME by both GC-FID and GC-MS. By contrast, d3-FAME coeluted with FAME on the polar GC column, and the use of the former as IS requires application of GC-MS. In the SIM mode, m/z 77 and 90 are suggested for d3-methyl esters of saturated fatty acids, whereas m/z 88 and 101 are recommended for ethyl esters of saturated fatty acids. These m/z values give either no or very low response for FAME and can thus be used for the analysis of FAME in food by GC-MS in the SIM mode. Fatty acids in sunflower oil and mozzarella cheese were quantified using five saturated FAEE as IS. Gravimetric studies showed that the transesterification procedure could be carried out without of loss of fatty acids. GC-EI/MS full scan analysis was suitable for the quantitative deter...Continue Reading

References

Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·K Eder

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Citations

Aug 13, 2011·Environmental Science and Pollution Research International·Natalie RosenfelderWalter Vetter
May 1, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Josef EckerGerhard Liebisch
Apr 18, 2013·Rapid Communications in Mass Spectrometry : RCM·Paul BendigWalter Vetter
Oct 16, 2014·Journal of Food Science·Stefanie KaffarnikWalter Vetter
May 22, 2016·Clinical Biochemistry·Natalie KummerChristophe P Stove
May 5, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T FierensG Vanermen
Aug 1, 2008·Applied Microbiology and Biotechnology·Young Soo KeumJeong Han Kim
Apr 24, 2018·Standards in Genomic Sciences·Mamadou BeyePierre-Edouard Fournier
Dec 6, 2018·International Journal of Systematic and Evolutionary Microbiology·Kiwoon BaekJang-Cheon Cho
Jul 22, 2018·International Journal of Systematic and Evolutionary Microbiology·Tao YuanGuixiang Peng
Jan 28, 2017·International Journal of Systematic and Evolutionary Microbiology·Amadou Hamidou TogoMatthieu Million
Oct 28, 2019·International Journal of Systematic and Evolutionary Microbiology·Dhiraj Kumar ChaudharyJaisoo Kim
Oct 10, 2020·International Journal of Systematic and Evolutionary Microbiology·Hyun-Ju NohYung Mi Lee
May 19, 2007·Analytical Chemistry·Saskia ThurnhoferWalter Vetter

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