Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs

Frontiers in Plant Science
Protiva Rani Das, Sherif M Sherif

Abstract

In recent years, RNA interference (RNAi) machinery has widely been explored by plant biologists for its potential applications in disease management, plant development, and germplasm improvement. RNAi-based technologies have mainly been applied in the form of transgenic plant generation and host-induced-gene-silencing (HIGS). However, the approval of RNAi-based transgenic plants has always been challenging due to the proclaimed concerns surrounding their impacts on human health and the environment. Lately, exogenous applications of double-stranded RNAs (dsRNAs), short interfering RNAs (siRNAs), and hairpin RNAs (hpRNAs) has emerged as another technology that could be regarded as more eco-friendly, sustainable, and publicly acceptable than genetic transformation. Inside the plant cell, dsRNAs can undergo several steps of processing, which not only triggers RNAi machinery but may also involve transitive and systemic silencing, as well as epigenetic modifications. Therefore, along with the considerations of proper exogenous applications of dsRNAs, defining their final destination into plant cells is highly relevant. In this review, we highlighted the significance of several factors that affect dsRNA-induced gene silencing, the fat...Continue Reading

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Citations

May 26, 2021·Environmental Science & Technology·Ke ZhangKimberly M Parker
Jun 3, 2021·Plants·Ullrich Dubiella, Irene Serrano
Jun 16, 2021·Frontiers in Plant Science·Demetrio MarcianòSilvia Laura Toffolatti
Jul 18, 2021·Journal of Food Science·Josemar Gonçalves de Oliveira FilhoMariana Buranelo Egea
Aug 21, 2021·Applied Microbiology and Biotechnology·Josemaría Delgado-Martín, Leonardo Velasco
May 5, 2021·Plant Biotechnology Journal·Gaetano GiudiceWalter Chitarra

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Methods Mentioned

BETA
transgenic
in vitro transcription

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