PMID: 3214473Nov 1, 1988Paper

Application of fluorescence densitometry to the study of net albumin uptake by the rabbit aortic wall up- and downstream of intercostal ostia

Atherosclerosis
P D Weinberg

Abstract

A method was developed for measuring levels of lissamine rhodamine B-labelled albumin in arterial walls with a spatial resolution of ca. 1 micron 3. The method was used to study variation in net macromolecular uptake up- and downstream of intercostal ostia in a small number of rabbit aortae. The physical and biological properties of the fluorescent tracer, including its stability, were compatible with its use in vivo. Tracer was injected into the ear vein of conscious rabbits. After 3 h the rabbits were anaesthetized and the thoracic aorta was fixed in situ at physiological pressure. The vessel was embedded in epoxy resin and longitudinal sections of the aortic wall were cut through the centre of the intercostal ostia. Photomicrographs were used to record fluorescence from these sections. The optical density across the photomicrographs was measured and related to the distribution of tracer in the wall. Mean intima-medial levels of tracer were greater downstream of ostia than upstream but were lower near the flow divider than further downstream. Higher levels of tracer were found in the adventitia than in the media and, in the latter, the distribution appeared to be related to the lamellar structure.

References

Feb 1, 1977·Circulation Research·R L BratzlerR S Lees
Jan 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M B StemermanR S Lees
Feb 16, 1971·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C G CaroR C Schroter
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Jul 1, 1962·The Journal of Clinical Investigation·L E DUNCANK BUCK

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Citations

May 11, 1999·Experimental and Molecular Pathology·Q JavedP D Weinberg
Feb 28, 1997·Atherosclerosis·B A Forster, P D Weinberg
Feb 25, 2003·Journal of Biomechanical Engineering·Benjamin A EwinsPeter D Weinberg
Apr 7, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Christina M WarboysPeter D Weinberg
Apr 28, 2004·Experimental Physiology·Tracey J Staughton, Peter D Weinberg
Mar 16, 2000·Experimental and Molecular Pathology·J P Richards, P D Weinberg
Oct 16, 2001·Experimental and Molecular Pathology·S E Barnes, P D Weinberg
Feb 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·A Sebkhi, P D Weinberg
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·B A Forster, P D Weinberg
Oct 16, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·S E Barnes, P D Weinberg
Mar 4, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·S E Barnes, P D Weinberg
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·T J StaughtonP D Weinberg
Jun 1, 2012·Cellular and Molecular Bioengineering·Christina M WarboysPeter D Weinberg

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