Application of fluorescence resonance energy transfer and magnetic twisting cytometry to quantify mechanochemical signaling activities in a living cell.

Science Signaling
Sungsoo Na, Ning Wang

Abstract

Mechanotransduction is the process by which living cells sense mechanical forces and then convert them into biochemical signaling. Recently, we showed that mechanical stress is transduced from the cell surface to remote cytoplasmic sites within 0.3 seconds, which is at least 40 to 50 times faster than soluble factor-induced signal transduction, and the sites of mechanotransduction colocalize with mechanical stress-induced microtubule displacements. These results suggest that mechanotransduction employs mechanisms different from those of soluble factor-induced signal transduction. Here, we describe a protocol that utilizes fluorescence resonance energy transfer (FRET) and a magnetic twisting cytometry (MTC) device to capture rapid mechanochemical signaling activities in living cells.

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Citations

Mar 5, 2009·Annals of Biomedical Engineering·Dennis DischerSheldon Weinbaum
Dec 18, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yingxiao Wang, Ning Wang
Apr 2, 2010·Journal of the Royal Society, Interface·Bo LiuYingxiao Wang
Feb 27, 2009·American Journal of Physiology. Cell Physiology·Sungsoo NaNing Wang
Apr 9, 2014·Journal of Biomechanics·Dominik DuscherGeoffrey C Gurtner
Oct 3, 2015·Methods : a Companion to Methods in Enzymology·R J MarjoramK Burridge
Apr 29, 2015·Biomaterials·Biran WangDebra T Auguste
Jan 8, 2014·Biochemical and Biophysical Research Communications·Seung Joon KimSungsoo Na
Dec 2, 2018·Frontiers in Physiology·Francesco BasoliAlberto Rainer

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