Application of Fluoro-Jade C in acute and chronic neurodegeneration models: utilities and staining differences.

Acta Histochemica Et Cytochemica
Ayuka Ehara, Shuichi Ueda

Abstract

Recent neuropathological studies have shown that Fluoro-Jade C (FJC), an anionic fluorescent dye, is a good marker of degenerating neurons. However, those studies have mostly examined acute rather than chronic models of neurodegeneration. We therefore compared FJC staining using the intrastriatal 6-hydroxydopamine (6-OHDA)-injected rat as an acute model and the zitter rat as a chronic model, as both show dopaminergic (DA) neurodegeneration. In the 6-OHDA-injected rat, FJC-positive neurons were found in the substantia nigra pars compacta (SNc) before the loss of tyrosine hydroxylase (TH)-positive DA neurons. In the zitter rat, FJC-labeled fibers were first detected at 1 month old (1M) and were considerably increased in the striatum at 4M, whereas FJC-labeled cell bodies were found at 4M, but not at 1M in the SNc. Furthermore, FJC-labeled neurons of the zitter rat showed TH-immunoreactivity in fibers, but little in cell bodies, while those from the 6-OHDA-injected rat showed TH-immunoreactivity even in the cell bodies. These results demonstrate that FJC is a useful tool for detecting chronically degenerating neurons, and suggest that intracellular substances bound to FJC may accumulate in the cell bodies from fibers at a slower r...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E WeberR Martin
Feb 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W D EldredS Yazulla
Feb 11, 2000·Toxicologic Pathology·L C Schmued, K J Hopkins
Oct 31, 2000·The Journal of Comparative Neurology·C L ZuchP C Bickford
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·T KuramotoT Serikawa
Jul 30, 2002·Progress in Brain Research·Shuichi UedaNoriyuki Koibuchi
Apr 13, 2006·Acta Neuropathologica·Kazuhiko NakadateShuichi Ueda
Mar 4, 2008·The European Journal of Neuroscience·Katharine HanrottSusan Wonnacott
Apr 17, 2008·The European Journal of Neuroscience·Lori K SmithGerlinde A Metz
Nov 18, 2008·Experimental Eye Research·Glyn ChidlowRobert J Casson

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Citations

Sep 22, 2011·Neuroscience Bulletin·Ghulam AbbasAhsana Dar
Apr 26, 2015·Physiology & Behavior·Dan XingLicai Zhang
Nov 29, 2011·The Journal of Comparative Neurology·David J Busch, Jennifer R Morgan
Apr 12, 2016·Scientific Reports·Sandra Gómez-LópezDiana Escalante-Alcalde
Jul 31, 2013·Hormones and Behavior·Huiling TangDonald G Stein
Nov 8, 2011·Neuroscience Letters·Ken-Ichi HashimotoKoichi Hirata
Nov 14, 2016·Translational Stroke Research·Kazim YigitkanliKlaus van Leyen
Mar 6, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mary B DailJanice E Chambers
Jan 21, 2020·The European Journal of Neuroscience·Ana Clara CristóvãoYoon-Seong Kim
Sep 25, 2017·Anatomical Science International·Ayuka EharaShiuchi Ueda
Feb 20, 2019·International Journal of Molecular Sciences·Hyeyoung KimJi Hyeon Ahn
Jan 4, 2019·Neurotoxicology·Sunil BhurtelDong-Young Choi
Apr 16, 2021·Current Biology : CB·Sabrina van HeukelumArthur S C França
Jul 3, 2021·International Journal of Molecular Sciences·Katarzyna ZalewskaFrederick R Walker
Sep 1, 2021·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Takuya IkenariTetsuji Mori
Sep 28, 2021·Acta Neuropathologica Communications·Joanna E PankiewiczMartin J Sadowski

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