Application of FT-IR spectroscopy for control of the medium composition during the biodegradation of nitro aromatic compounds.

Journal of Industrial Microbiology & Biotechnology
Mara GrubeBaiba Limane

Abstract

Previous studies showed that cabbage leaf extract (CLE) added to the growth medium can noticeably promote the degradation of nitro aromatic compounds by specific consortium of bacteria upon their growth. For further development of the approach for contaminated soil remediation it was necessary to evaluate the qualitative and/or quantitative composition of different origin CLE and their relevance on the growth of explosives-degrading bacteria. Six CLE (different by species, cultivars and harvesting time) were tested and used as additives to the growth medium. It was shown that nitro aromatic compounds can be identified in the FT-IR absorption spectra by the characteristic band at 1,527 cm(-1), and in CLE by the characteristic band at 1,602 cm(-1). The intensity of the CLE band at 1,602 cm(-1) correlated with the concentration of total nitrogen (R2=0.87) and decreased upon the growth of bacteria. The content of nitrogen in CLE differed (0.22-1.00 vol.%) and significantly influenced the content of total carbohydrates (9.50-16.00% DW) and lipids [3.90-9.90% dry weight (DW)] accumulated in bacterial cells while the content of proteins was similar in all samples. Though this study showed quantitative differences in the composition of...Continue Reading

References

Sep 23, 2000·Journal of Microbiological Methods·F OrsiniS M Doglia
May 19, 2001·Water Research·J D Rodgers, N J Bunce
Aug 21, 2008·Journal of Industrial Microbiology & Biotechnology·Olga MuterDzidra Zarina

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Citations

Aug 21, 2008·Journal of Industrial Microbiology & Biotechnology·Olga MuterDzidra Zarina
Oct 19, 2017·Environmental Science and Pollution Research International·Meenakshi Srivastava, Arun Kumar Mishra
May 29, 2018·Environmental Science and Pollution Research International·Virbala Sharma, Deepak Pant
Jun 20, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Falguni Patel, B Lakshmi

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