Application of fungal and bacterial production methodologies to decomposing leaves in streams.

Applied and Environmental Microbiology
K Suberkropp, H Weyers

Abstract

As leaves enter woodland streams, they are colonized by both fungi and bacteria. To determine the contribution of each of these microbial groups to the decomposition process, comparisons of fungal and bacterial production are needed. Recently, a new method for estimating fungal production based on rates of [(sup14)C]acetate incorporation into ergosterol was described. Bacterial production in environmental samples has been determined from rates of [(sup3)H]leucine incorporation into protein. In this study, we evaluated conditions necessary to use these methods for estimating fungal and bacterial production associated with leaves decomposing in a stream. During incubation of leaf disks with radiolabeled substrates, aeration increased rates of fungal incorporation but decreased bacterial production. Incorporation of both radiolabeled substrates by microorganisms associated with leaf litter was linear over the time periods examined (2 h for bacteria and 4 h for fungi). Incorporation of radiolabeled substrates present at different concentrations indicated that 400 nM leucine and 5 mM acetate maximized uptake for bacteria and fungi, respectively. Growth rates and rates of acetate incorporation into ergosterol followed similar pattern...Continue Reading

References

Feb 1, 1993·Applied and Environmental Microbiology·M O Gessner, E Chauvet
Oct 1, 1993·Applied and Environmental Microbiology·K SuberkroppE Chauvet

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Citations

Nov 13, 2007·Microbial Ecology·Samir Damare, Chandralata Raghukumar
Oct 27, 2001·Applied and Environmental Microbiology·K Suberkropp
Dec 7, 2005·FEMS Microbiology Ecology·Janna PietikäinenErland Bååth
Dec 18, 2007·Applied and Environmental Microbiology·Vladislav GulisAmy D Rosemond
Feb 12, 2008·Microbial Ecology·Mayura A ManerkarFelix Bärlocher
Jan 5, 2006·Applied and Environmental Microbiology·Nanna Buesing, Mark O Gessner
Sep 4, 2004·Applied and Environmental Microbiology·Cláudia Pascoal, Fernanda Cássio
Apr 12, 2005·The New Phytologist·Claudia I CzimczikSusan E Trumbore
Aug 9, 2006·Journal of Medical Entomology·Michael G Kaufman, Edward D Walker
Dec 14, 2005·Applied and Environmental Microbiology·E Chauvet, K Suberkropp

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