Application of gene differential expression technology in the mechanism studies of nature product-derived drugs

Expert Opinion on Biological Therapy
Shan-Li Chen, Jian-Guo Jiang

Abstract

Traditional Chinese medicine (TCM) efficacy is a comprehensive result of influences of complex chemical components contained in TCM having on different targets at gene, cell, and tissue levels. Multi-component, multi-target, and multi-level characteristics cause barriers to molecular mechanism research of TCM. The emergence of gene differential expression technology provides a strong arm for TCM study, which enables researchers to assay target-genetic differential expression after TCM treatment in high-throughput manner. Focused and comprehensive literature and references are undertaken to introduce the technologies at gene level and at protein level. The former includes genechip, semi-quantitative/fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction), northern blot, etc. The latter includes western blot, immunohistochemistry, ELISA (enzyme-linked immunosorbent assay), etc. The literature reviews applications of technologies above in TCM mechanisms research over the past decade and also makes a comparison of different technologies for reference. Although gene differential expression technology has obvious advantages in studying the mechanism of TCM, biotechnology should be further explored to meet t...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J RenartG R Stark
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Aug 1, 1988·Biochemical Pharmacology·G W De VriesL A Wheeler
Feb 1, 1987·Molecular and Biochemical Parasitology·E HempelmannU Gröschel-Stewart
Jun 1, 1995·Neuron·N J Sucher, D L Deitcher
Sep 23, 1997·Biochemical and Biophysical Research Communications·C G KevilJ S Alexander
Jul 24, 1998·Trends in Biotechnology·M SchenaR W Davis
Apr 22, 1999·Environmental Health Perspectives·J F Medlin
Aug 17, 1999·Journal of Clinical Microbiology·A AbeM Kohara
Jan 19, 2000·Drug Discovery Today·I Drews
Feb 1, 2000·Drug Discovery Today·G G Lennon
Apr 25, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·W D PennieI Kimber
Sep 7, 2001·Plant Molecular Biology Reporter·A Nebenfuhr, T L Lomax
Jul 18, 2002·Nature Reviews. Drug Discovery·Allen D Roses
Apr 23, 2003·Trends in Pharmacological Sciences·Silvia MandelMoussa B H Youdim
May 26, 2004·Trends in Biotechnology·Aldo RodaMassimo Guardigli
Jul 12, 2005·Veterinary Pathology·J A Ramos-Vara
Sep 28, 2005·Trends in Pharmacological Sciences·Wen-Yue Jiang
Feb 8, 2006·Molecular Aspects of Medicine·Mikael KubistaNeven Zoric
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Janet S Duerr
Aug 12, 2009·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Ping LiYue-wen Gong
Mar 20, 2010·Methods in Molecular Biology·Fadia Haddad, Kenneth M Baldwin
Nov 17, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Bing HuQin Du
Oct 11, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ji-Hae YoonSeong-Gene Lee
Dec 16, 2011·Virology Journal·Wen HeBin Gao
Mar 6, 2012·BMC Genomics·Zhongxue ChenRichard H Scheuermann

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