Application of headspace solid phase dynamic extraction gas chromatography/mass spectrometry (HS-SPDE-GC/MS) for biomonitoring of n-heptane and its metabolites in blood

Toxicology Letters
Bernd RossbachStephan Letzel

Abstract

Solid phase dynamic extraction (SPDE) is an innovative sample preparation and enrichment technique in connection with gas chromatography (GC). Using SPDE, we developed a method for simultaneous determination of n-heptane and its mono-oxygenated metabolites heptane-4-one, 3-one, 2-one, 4-ol, 3-ol, 2-ol, and 1-ol in blood. After adjustment of various extraction and desorption parameters, method validation resulted in limits of detection (LOD) between 0.006 (heptane-4-one) and 0.021mg/L (heptane-1-ol). Intra-assay coefficients of variation ranged between 4.8% and 20.8% while relative recovery ranged between 100% and 117% (spiked concentration 0.128mg/L, n=8). The method was applied to blood samples, which have been collected from 20 volunteers after controlled inhalative exposure to 167, 333, and 500ppm n-heptane. After 3h of exposure, n-heptane and heptane-2-one were detectable in all samples in concentrations ranging up to 2.903 and 0.495mg/L, while the concentrations of the remaining analytes were closer to the respective LOD or even below. A significant linear relationship with ambient exposure (R(2)=0.701, p<0.001, n=55) was found for n-heptane in blood, which could be helpful for evaluation of biological exposure limits in f...Continue Reading

References

May 8, 2014·International Journal of Occupational and Environmental Health·Fabian Melchior GersterNancy Brenna Hopf
Sep 5, 2015·Bioanalysis·Alexandra GonçalvesMário Barroso
Jul 28, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stefano DugheriNicola Mucci

Citations

Apr 1, 1986·Archives of Toxicology·L PerbelliniG B Bartolucci
Oct 1, 1980·Critical Reviews in Toxicology·P S SpencerB Veronesi
Feb 24, 2001·Fresenius' Journal of Analytical Chemistry·J Lipinski
May 14, 2003·Forensic Science International : Synergy·Frank MusshoffBurkhard Madea
Nov 2, 2004·Journal of Chromatographic Science·Carlo BicchiPatrizia Rubiolo
Mar 15, 2006·Journal of Chromatography. a·Maik A JochmannTorsten C Schmidt
Jan 1, 1997·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· A Sturaro G Parvoli L Doretti F Valentini G Gori And G B Bartolucci

Related Concepts

Metabolic Process, Cellular
Biochemical Pathway
Ethanol
n-hexane
Isoheptanes
Heptanes
Diketone
Dimethyl ether
Gas Chromatography-Mass Spectrometry
Uptake

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