Application of HEMA on intact mouse skin--effects on the immune system

Contact Dermatitis
E Sandberg, Ulf Dahlgren

Abstract

Previously, we have shown that 2-hydroxyethylmethacrylate (HEMA) can bind to protein and that autoantibodies were induced in mice by immunization with a self-protein in vitro conjugated with HEMA. The present study aimed to develop a model for HEMA-induced sensitivity by the application of the substance on intact skin. Female BALB/c mice were painted on the dorsum of each ear with 50% HEMA in vehicle twice a week for 6 weeks. The anti-CD3epsilon-stimulated lymph node production and the spontaneous spleen-cell production of the cytokines interleukin (IL)-2, IL-4, IL-6, IL-10 and interferon-gamma were assessed by enzyme-linked immunosorbent assay. In another experiment, the cytokines were followed after subcutaneous HEMA injections. Animals painted with HEMA had a significantly higher IL-6 production by anti-CD3epsilon-stimulated lymph node cells and significantly suppressed IL-10 production by spleen cells compared to vehicle-treated mice. This correlated to some extent with the spontaneous spleen-cell production induced by subcutaneously administered HEMA. An injection of 20 micromol of HEMA induced an increased production of IL-6, while injection of 40 micromol depressed both IL-6 and IL-10 production. Although there was no si...Continue Reading

References

Mar 1, 1992·Dental Materials : Official Publication of the Academy of Dental Materials·N Nakabayashi, K Takarada
Apr 1, 1985·Contact Dermatitis·S Clemmensen
Jul 1, 1995·The Journal of Experimental Medicine·D J BergD Rennick
May 1, 1994·The Journal of Experimental Medicine·T A FergusonT S Griffith
Feb 18, 1998·Toxicology and Applied Pharmacology·T RustemeyerR J Scheper
Nov 21, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P PiiriläH Nordman
Nov 11, 1999·Journal of Biomedical Materials Research·W GeurtsenG Leyhausen
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B WangD N Sauder
Jun 20, 2001·American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society·L KanervaA M Vuorela
Aug 8, 2001·The Australasian Journal of Dermatology·D Koutis, S Freeman
Nov 21, 2001·Toxicology and Applied Pharmacology·T RustemeyerR J Scheper
Aug 31, 2002·Journal of Dental Research·E SandbergU I Dahlgren
Jan 30, 2003·Journal of Dentistry·F-X ReichlR Hickel
Apr 21, 2004·Journal of the American Academy of Dermatology·Elliot WeissDaniel Nathan Sauder
May 7, 2004·Contact Dermatitis·Kristiina AlankoLasse Kanerva
Oct 6, 2005·European Journal of Oral Sciences·Elisabeth SandbergUlf I Dahlgren

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Citations

Mar 18, 2011·European Journal of Oral Sciences·Jennie Andersson, Ulf Dahlgren
Jul 6, 2011·European Journal of Oral Sciences·Jennie Andersson, Ulf Dahlgren
Apr 27, 2017·European Journal of Oral Sciences·Bo W NilsenVibeke B Michelsen
Sep 3, 2010·Journal of Dental Research·J Andersson, U Dahlgren
May 11, 2018·Clinical and Experimental Dental Research·Sara AlizadehgharibUlf Dahlgren
Dec 13, 2017·Acta Biomaterialia Odontologica Scandinavica·Sara AlizadehgharibUlf Dahlgren
Jun 17, 2020·Clinical and Experimental Dental Research·Sara AlizadehgharibKarin Christenson
May 31, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Renke PerdunsWerner Geurtsen

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