Application of HER2 CISH pharmDX for DNA Ploidy Determination

Applied Immunohistochemistry & Molecular Morphology : AIMM
Mai HeMargaret Steinhoff

Abstract

Products of conception (POC) are encountered daily in general pathology practice. The molar workup is an important part of POC examination. Ploidy analysis, expressed as DNA index (DI), is part of the pathologic workup of molar pregnancy. For the past decade, chromogenic in situ hybridization (CISH) has become a popular way to detect HER2 gene amplification. Current study aims to determine whether HER2 CISH dual-color assay can be used to determine DI in POCs. Twenty-two POC cases were chosen from the departmental archives, including 6 complete hydatidiform mole (CM), 10 partial mole (PM), and 6 hydropic POC (HP). CISH assay was performed using the HER2 CISH PharmDx Kit (SK109; Dako). This kit generates red (HER2) and blue (CEN-17) chromogenic signals on the same tissue section. In the 10 triploid PM cases, CISH generated HER2 signal value of 2.925±0.19. Nine cases (90%) had values within this range, except 1 case (2.5). In diploid cases, CISH generated HER2 signal value of 2.063±0.19. Results from 11 (91.7%) cases fell within this range, except 1 HP case (2.35). Sensitivity is 90%, specificity 91.6%, and overall accuracy 90.9%. The current study is the first one that demonstrates HER2/CEN-17 dual-color CISH can be used for mic...Continue Reading

References

Jun 18, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jianxin ZhaoZuorong Shi
Sep 19, 2009·American Journal of Clinical Pathology·Frédérique Penault-LlorcaMarc van de Vijver
Dec 25, 2012·Virchows Archiv : an International Journal of Pathology·Isabella GrinschglBarbara Guertl

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