Application of high-resolution MS for development of peptide and large-molecule drug candidates

Bioanalysis
John F KellieMatthew E Szapacs

Abstract

For quantitative bioanalysis utilizing MS, the instrument of choice is typically a triple quadruple mass spectrometer. However, advances in high-resolution MS have allowed sensitivity and dynamic ranges to approach that of triple quadrupole instruments. A matrix-free protein digest, a digested therapeutic protein and the intact peptide therapeutic liraglutide were each analyzed on high-resolution and triple quadrupole mass spectrometers with data compared. Samples from a mouse PK study with liraglutide were analyzed using the two different instruments, and equivalent PK exposure data were demonstrated. High-resolution and triple quadrupole mass spectrometers can generate data resulting in identical PK parameters from an in-life sample set, thus giving confidence in either technique in support of biotherapeutic PK exposure studies.

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Citations

Jul 16, 2016·Bioanalysis·Brian Rago, Andre Negahban
Dec 6, 2017·Bioanalysis·Yingfei YanHongliang Jiang
Jul 2, 2019·Biomedical Chromatography : BMC·Lijuan KangWenying Jian
Dec 15, 2016·Bioanalysis·Sankeetha Nadarajah

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Phoenix WinNonlin
TargetLynx

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