Application of high-speed counter-current chromatography coupled with high-performance liquid chromatography-diode array detection for the preparative isolation and purification of hyperoside from Hypericum perforatum with online purity monitoring

Journal of Chromatography. a
Tingting ZhouYutian Wu

Abstract

Following preparative isolation and purification by high-speed counter-current chromatography (HSCCC), the collected fractions were generally analyzed by high-performance liquid chromatography (HPLC) to determine the relative purities of each fraction. Our paper reports for the first time a preparative isolation-purity detection hyphenated system: online coupling of HSCCC with high-performance liquid chromatography-diode array detection (HSCCC-HPLC-DAD). The introduction of online purity analysis in HSCCC has dramatically improved the efficiency of this technique by overcoming the drawbacks of post analysis in HSCCC isolation. The effluent from the outlet of HSCCC was splitted into two parts: one was collected, while the other was introduced directly into an HPLC-DAD system for purity analysis through a switch valve. Therefore, the purities of the obtained fractions from HSCCC were monitored, and fractions with high purities were collected. This strategy has been successfully demonstrated with the preparative isolation and purification of hyperoside from Hypericum perforatum (St. Jone's Wort); a model of TBE-300A HSCCC was used to isolate and separate hyperoside from H. perforatum with a two-phase solvent system composed of eth...Continue Reading

References

Jun 8, 2001·Trends in Pharmacological Sciences·G Di CarloA A Izzo

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Citations

Mar 23, 2011·Journal of Agricultural and Food Chemistry·Gang ZhaoLianxin Peng
May 24, 2008·Natural Product Reports·Otto Sticher
Jun 12, 2013·Analytical and Bioanalytical Chemistry·Thomas MichelClaire Elfakir
Dec 9, 2015·Journal of Chromatography. a·Yaping WangLinfang Huang
Sep 26, 2008·Biomedical Chromatography : BMC·Andreana N AssimopoulouVassilios P Papageorgiou
Jul 11, 2008·Journal of Separation Science·Shao LiuYi-Zeng Liang
Nov 30, 2021·Journal of Medicinal Chemistry·Jinle TangHao Huang

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