Application of high-throughput mini-bioreactor system for systematic scale-down modeling, process characterization, and control strategy development

Biotechnology Progress
Vijay JanakiramanYao-Ming Huang

Abstract

High-throughput systems and processes have typically been targeted for process development and optimization in the bioprocessing industry. For process characterization, bench scale bioreactors have been the system of choice. Due to the need for performing different process conditions for multiple process parameters, the process characterization studies typically span several months and are considered time and resource intensive. In this study, we have shown the application of a high-throughput mini-bioreactor system viz. the Advanced Microscale Bioreactor (ambr15(TM) ), to perform process characterization in less than a month and develop an input control strategy. As a pre-requisite to process characterization, a scale-down model was first developed in the ambr system (15 mL) using statistical multivariate analysis techniques that showed comparability with both manufacturing scale (15,000 L) and bench scale (5 L). Volumetric sparge rates were matched between ambr and manufacturing scale, and the ambr process matched the pCO2 profiles as well as several other process and product quality parameters. The scale-down model was used to perform the process characterization DoE study and product quality results were generated. Upon com...Continue Reading

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Citations

Sep 29, 2018·Current Protocols in Protein Science·Molly HunterMark Fox
Oct 24, 2018·Biotechnology Journal·Viktor SandnerJarka Glassey
Dec 5, 2018·Biotechnology and Bioengineering·Mohamed MahameedBoaz Tirosh
Feb 7, 2019·Critical Reviews in Biotechnology·André GuerraJarka Glassey
Jul 28, 2017·Biotechnology Progress·M Lourdes Velez-SuberbieEli Keshavarz-Moore
Jul 19, 2019·Biotechnology and Bioengineering·Emmanuel AnaneMariano Nicolas Cruz Bournazou
Sep 18, 2020·Micromachines·Spyridon AchinasGerrit Jan Willem Euverink
Sep 19, 2020·Engineering in Life Sciences·Katrin PaulChristoph Herwig
Sep 29, 2019·Cytotechnology·Peifeng ChenFrank Arnold
Nov 2, 2021·Frontiers in Bioengineering and Biotechnology·Julian SchmitzAlexander Grünberger

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