Feb 1, 1986

Application of immobilized heparins for isolation of human antithrombin III

Biotechnology and Bioengineering
G MitraI Mitra

Abstract

Immobilized heparins were prepared by six different methods, and these were utilized for affinity purification of human antithrombin III (AT-III). Affinity support capacities (mg AT-III/g support) were strongly influenced by immobilized active heparin concentrations. In the temperature range 5-37 degrees C, colder temperatures favored affinity adsorption of AT-III as well as nonspecific interactions of all proteins. For representative human-plasma-derived feed solutions the selectivity for AT-III on the affinity support was dependent on relative concentrations of non-AT-III proteins as well as the specific mode of adsorption and elution (batch/continuous).

  • References4
  • Citations4

Citations

Mentioned in this Paper

Antithrombin III, Human
Heparin
Adsorption
Heparin, porcine
Ataxia Telangiectasia
Antithrombin III Deficiency
Chromatography, Affinity

Related Feeds

Ataxia telangiectasia

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.