PMID: 11905039Mar 22, 2002Paper

Application of immunohistochemistry for forensic pathological diagnosis: finding of human brain in forensic autopsy

Nihon hōigaku zasshi = The Japanese journal of legal medicine
Ichiro Nakasono

Abstract

We researched the application of immunohistochemistry for the purpose of establishing forensic pathological diagnoses. In the present study, we examined the induction and expression of heat shock protein (HSP), oxygen regulated protein (ORP), inducible nitric oxide synthase (iNOS), excitatory amino acid transporter 2 (EAAT2) and apolipoprotein E (apo E) in the human brain using forensic autopsy cases as our subjects. Hypoxic/ischemic brain damage. In cases of longer survival and with a history of hypoxic attacks, the proteins HSP and ORP were found in the parieto-occipital lobe and hippocampus. And we are able to observe a weak stain for EAAT2 in almost all asphyxia deaths. Traumatic brain injury (TBI). In traumatic brain injury (TBI), the prolonged induction of iNOS was demonstrated in the neutrophils, microglia/macrophage, and vascular smooth muscle cells in the traumatized brain. Apo E was identified with neurons in the traumatized cortical hemisphere from only a two-hour survival case to long survival cases. To the contrary, there was no positive apo E staining in the contralateral cortical hemisphere at all. In one one-hour survival case, a weak stain for EAAT2 was observed, but intensive expression of EAAT2 was observed f...Continue Reading

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