PMID: 8946010Nov 13, 1996Paper

Application of immunoprinting for assessment of fibroblast secretory heterogeneity

Journal of Immunological Methods
A I Dongari-Bagtzoglou, J L Ebersole

Abstract

Fibroblasts are capable of secreting a number of inflammatory mediators and cytokines and may exhibit a marked heterogeneity in this capacity. The relative frequency of cytokine-secreting fibroblasts in chronic inflammatory connective tissue disorders may affect the amount of these molecules secreted locally and dictate the intensity or chronicity of the disease process. We have devised a simple, in situ immunodetection method for assessment of the frequency of actively secreting cells in adherent cell cultures. Our technique is based on the principle of immunoprinting and is coupled with an enzyme-linked immunodetection system. The methodology, sensitivity, specificity and accuracy of this technique are described.

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S D KoA S Narayanan
Dec 1, 1992·Journal of Dental Research·L Häkkinen, H Larjava
Feb 9, 1991·Lancet·S DerdakR Phipps
Mar 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K TakahashiT Goto
Sep 1, 1989·European Journal of Immunology·B J SkidmoreJ M Chiller
Jul 1, 1989·American Journal of Respiratory Cell and Molecular Biology·R P PhippsM E Felch
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S B MizelS E Mergenhagen
Sep 1, 1994·Journal of Periodontal Research·C R IrwinM W Ferguson
Sep 1, 1994·Clinical Immunology and Immunopathology·K M FriesR P Phipps
Nov 1, 1994·American Journal of Respiratory Cell and Molecular Biology·K M FriesR P Phipps
Dec 1, 1993·Journal of Periodontology·L T Hou, J A Yaeger
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T MatsusakaS Akira
Feb 1, 1996·Journal of Periodontal Research·A I Dongari-Bagtzoglou, J L Ebersole

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Citations

Jul 15, 2000·Oral Microbiology and Immunology·A Dongari-BagtzoglouI B Lamster
Sep 15, 1998·Journal of Periodontology·A I Dongari-Bagtzoglou, J L Ebersole
Jan 13, 2000·Journal of Periodontology·R S Morton, A I Dongari-Bagtzoglou

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