Application of ion-exchange cartridge clean-up in food analysis. V. Simultaneous determination of sulphonamide antibacterials in animal liver and kidney using high-performance liquid chromatography with ultraviolet and mass spectrometric detection

Journal of Chromatography. a
Y ItoH Nagase


A simple, rapid, and reliable method for the determination of residual sulphonamide antibacterials (SAs) (sulfadiazine, sulfamerazine, sulfadimidine, sulfamethoxypiridazine, sulfisozole, sulfamonomethoxine, sulfamethoxazole, sulfisoxazole, sulfadimethoxine, and sulfaquinoxaline) in animal liver and kidney was developed using a combination of clean-up on a Bond Elut PSA cartridge and HPLC with UV detection. The SAs were extracted with ethyl acetate and then dissolved in 5 ml of 50 v/v% ethyl acetate-n-hexane after being evaporated to dryness. For clean-up of the crude sample, the resuspended extract was applied to a Bond Elut PAS (primary/secondary amine cartridge), and then SAs were eluted from the cartridge using 5 ml of 20 v/v% acetonitrile-0.05 M ammonium formate before being analysed by HPLC. Recoveries of the SAs at the levels of 0.5 and 0.1 microg/g were 70.8-98.2%, the rerative standard deviation were less than 7.0%, and the detection limits were 0.03 microg/g. The present analysis method of SAs in animal kidney and liver using HPLC with a clean-up procedure was demonstrated to be highly applicable to the direct LC-MS-MS analysis without any modification.


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Mar 21, 2006·Food Additives and Contaminants·S WangI Kennedy
Jan 22, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Mizuka FujitaHirotaka Obana
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Mar 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ayman H KamelFelismina T C Moreira
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Jan 28, 2021·Journal of Chromatographic Science·Adel M MichaelChristine K Nessim

Related Concepts

High Pressure Liquid Chromatography Procedure
Ion-Exchange Chromatography Procedure
Food Analysis
Spectrophotometry, Ultraviolet
Mass Spectrometry

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