Application of isotope dilution analysis for the evaluation of extraction conditions in the determination of total selenium and selenomethionine in yeast-based nutritional supplements

Journal of Agricultural and Food Chemistry
L Hinojosa ReyesAlfredo Sanz-Medel

Abstract

Isotope dilution analysis (IDA) has been used to quantify total selenium, total solubilized selenium, and the selenomethionine (SeMet) amount in yeast and yeast-based nutritional supplements after acid microwave digestion and different enzymatic extraction procedures. For this purpose, both a (77)Se-enriched SeMet spike, previously synthesized and characterized in our laboratory, and a (77)Se(VI) spike were used. In the analysis of the nutritional supplements, the SeMet spike was added to the sample and extracted under different conditions, and the (78)Se/(77)Se and (80)Se/(77)Se isotope ratios were measured as peak area ratios after high-performance liquid chromatography (HPLC) separation and inductively coupled plasma mass spectrometry (ICP-MS) detection. The formation of SeH(+) and mass discrimination were corrected using a natural SeMet standard injected every three samples. Similarly, total solubilized selenium was measured in the extracts after enzymatic hydrolysis using the (77)Se-enriched SeMet as a spike by direct nebulization without a chromatographic separation. To establish a mass balance, total selenium was also determined by IDA-ICP-MS on the yeast tablets after microwave digestion using (77)Se(VI) as a spike. Res...Continue Reading

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Citations

Feb 3, 2009·Analytica Chimica Acta·Zoyne Pedrero, Yolanda Madrid
Apr 10, 2015·Biological Trace Element Research·Sheena FaganRichard Murphy
Apr 9, 2013·Food Chemistry·Jorge Moreda-PiñeiroPilar Bermejo-Barrera
May 1, 2015·Asian-Australasian Journal of Animal Sciences·Peter F Surai, Vladimir I Fisinin
Apr 22, 2015·Critical Reviews in Food Science and Nutrition·Jorge Moreda-PiñeiroPilar Bermejo-Barrera
Jan 1, 2018·The Journal of Poultry Science·Peter F SuraiOksana A Velichko

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