Application of Lectin Microarrays for Biomarker Discovery.

ChemistryOpen
Kai DangAirong Qian

Abstract

Many proteins in living organisms are glycosylated. As their glycan patterns exhibit protein-, cell-, and tissue-specific heterogeneity, changes in the glycosylation levels could serve as useful indicators of various pathological and physiological states. Thus, the identification of glycoprotein biomarkers from specific changes in the glycan profiles of glycoproteins is a trending field. Lectin microarrays provide a new glycan analysis platform, which enables rapid and sensitive analysis of complex glycans without requiring the release of glycans from the protein. Recent developments in lectin microarray technology enable high-throughput analysis of glycans in complex biological samples. In this review, we will discuss the basic concepts and recent progress in lectin microarray technology, the application of lectin microarrays in biomarker discovery, and the challenges and future development of this technology. Given the tremendous technical advancements that have been made, lectin microarrays will become an indispensable tool for the discovery of glycoprotein biomarkers.

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Citations

Jan 17, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ruben Warkentin, David H Kwan
May 3, 2021·Molecular Immunology·Janaina Macedo-da-SilvaGiuseppe Palmisano
May 21, 2021·Frontiers in Immunology·Siting LiChaojun Hu
Jul 13, 2021·Frontiers in Immunology·Xiaoli ZengChaojun Hu

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Methods Mentioned

BETA
glycosylation
Assay
antibody sandwich
Antibody
immunoprecipitation
ELISA
PCR
surface plasmon resonance
galactosylation
flow cytometry

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