Application of liposomes to sonoporation

Ultrasound in Medicine & Biology
Junru WuMercedes Ricń

Abstract

A method to prepare liposomes is presented. Liposomes made in our laboratory were characterized acoustically and optically. The phase velocity and attenuation of liposomes in suspension (concentration = 10(9)/mL) were measured, ranging from 2 to 14 MHz, using ultrasound spectroscopy. Anti-rabbit IgG conjugated with Alexafluor 647 was delivered into Jurkat cells in suspension, using the liposomes, by 10 % duty cycle ultrasound tonebursts of 2.2 MHz (the in situ spatial peak-pressure amplitude = 80 W/cm2) with an efficiency of 13 %. It has been experimentally shown that liposomes may be an alternative stable agent to Optison for delivering macromolecules into cells.

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Citations

Feb 6, 2013·Theranostics·Steven P WrennPeter A Lewin
Dec 12, 2012·Biomedical Microdevices·A L BernassauD R S Cumming
Apr 7, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·S RizzitelliE Terreno
Aug 30, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fang YangJunru Wu
Jan 3, 2019·The Journal of the Acoustical Society of America·Dong MaJunru Wu

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