Application of liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry in the quantitative analysis of glyburide (glibenclamide) in human plasma

Rapid Communications in Mass Spectrometry : RCM
L RamosF Tse

Abstract

Glyburide (glibenclamide) is widely prescribed in the treatment of Type II diabetes. A validated liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (LC/APCI-MS/MS) method for the determination of glyburide is reported. The method uses a stable isotope labeled glyburide as the internal standard. Subsequent to acetonitrile protein precipitation, the supernatant was directly (unfiltered) injected onto the LC column (retention time approximately 3 min) for analysis. A lower limit of quantification (LLOQ) of 1.01 ng/mL was attained for the human plasma assay. The method was fast, specific, and exhibited excellent ruggedness. It was successfully applied to the analysis of clinical samples from patients dosed with glyburide.

References

Jul 12, 1996·Journal of Chromatography. B, Biomedical Applications·J R Valdes Santurio, E González Porto
Apr 21, 1998·Journal of Pharmaceutical Sciences·E Brewer, J Henion
Apr 29, 1998·European Journal of Clinical Pharmacology·A JönssonA Melander
Jan 12, 1999·Journal of Pharmaceutical and Biomedical Analysis·M CarrascalJ Abian

Citations

Jul 14, 2007·Journal of Pharmaceutical and Biomedical Analysis·Hiren N MistriPranav S Shrivastav
Aug 31, 2002·Rapid Communications in Mass Spectrometry : RCM·Simone CristoniFederico Guidugli
Jun 27, 2000·Rapid Communications in Mass Spectrometry : RCM·R Bakhtiar, F L Tse

Related Concepts

Calibration
Liquid Chromatography
Neogluconin
Hypoglycemic Effect
Mass Spectrometry

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