Application of long PCR method of identification of variations in nucleotide sequences among varicella-zoster virus isolates.

Journal of Clinical Microbiology
M TakayamaY Kameoka

Abstract

Restriction fragment length polymorphism (RFLP) analysis of whole viral DNA of varicella-zoster virus (VZV) requires the time-consuming and laborious preparation of a large amount of purified viral DNA. RFLP analysis of small DNA fragments amplified by PCR was developed as an alternative method. However, its use has been limited because of the small number of variations in VZV. To overcome these drawbacks and to identify variations in VZV, we developed an RFLP analysis method combined with the long PCR method which has recently been developed for the amplification of DNA fragments between 5 and 35 kb in length. We amplified three DNA regions ranging from 6.8 to 11.4 kb and demonstrated that RFLP analyses of these regions allowed for the classification of 40 VZV isolates in Japan into 17 groups. One-fourth of the isolates contained a nucleotide difference of C versus T, which abolished the StyI site at position 76530; this alteration was linked to the reported PstI site polymorphism at position 69349 (nucleotide positions are based on those of strain Dumas). Nucleotide sequence variation in the examined regions among VZV isolates in Japan was estimated at roughly less than 0.05%, confirming the previously proposed idea that VZV ...Continue Reading

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Citations

Aug 10, 2000·American Journal of Respiratory and Critical Care Medicine·C T CowlM R Migden
Mar 26, 2004·Journal of Medical Virology·Michael J CarrBrendan Crowley
Mar 11, 2003·Journal of Medical Virology·W Barrett-MuirJ Breuer
Jun 23, 2006·Reviews in Medical Virology·Mark Quinlivan, Judith Breuer
Jul 13, 2007·Clinical and Vaccine Immunology : CVI·Marjaleena KoskiniemiVladimir N Loparev
Feb 29, 2000·The Pediatric Infectious Disease Journal·N TakayamaJ Takita
Oct 22, 2002·Journal of Virology·Yasuyuki GomiKoichi Yamanishi
Nov 30, 2011·PloS One·Daniel P DepledgeJudith Breuer
Dec 1, 2006·Journal of Clinical Microbiology·Vladimir LoparevAnne-Marie Fillet
Oct 9, 1999·Clinical Microbiology Reviews·M Arens
Apr 9, 2004·Journal of Clinical Microbiology·Paul A CampsallEva E Thomas
Sep 24, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Peter Norberg

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