Application of marrow mesenchymal stem cell-derived extracellular matrix in peripheral nerve tissue engineering

Journal of Tissue Engineering and Regenerative Medicine
Yun GuJianhui Gu

Abstract

To advance molecular and cellular therapy into the clinic for peripheral nerve injury, modification of neural scaffolds with the extracellular matrix (ECM) of peripheral nerves has been established as a promising alternative to direct inclusion of support cells and/or growth factors within a neural scaffold, while cell-derived ECM proves to be superior to tissue-derived ECM in the modification of neural scaffolds. Based on the fact that bone marrow mesenchymal stem cells (BMSCs), just like Schwann cells, are adopted as support cells within a neural scaffold, in this study we used BMSCs as parent cells to generate ECM for application in peripheral nerve tissue engineering. A chitosan nerve guidance conduit (NGC) and silk fibroin filamentous fillers were respectively prepared for co-culture with purified BMSCs, followed by decellularization to stimulate ECM deposition. The ECM-modified NGC and lumen fillers were then assembled into a chitosan-silk fibroin-based, BMSC-derived, ECM-modified neural scaffold, which was implanted into rats to bridge a 10 mm-long sciatic nerve gap. Histological and functional assessments after implantation showed that regenerative outcomes achieved by our engineered neural scaffold were better than tho...Continue Reading

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Citations

Dec 15, 2017·Chinese Medical Journal·Bao-Guo JiangPei-Xun Zhang
Feb 1, 2019·Expert Opinion on Biological Therapy·Sara Sayad-FathiArash Zaminy
Feb 2, 2018·Biotechnology & Genetic Engineering Reviews·Behnaz BakhshandehZarrintaj Ziraksaz
Jun 5, 2018·Neural Regeneration Research·Nitesh P PatelJason H Huang
Jan 10, 2017·International Journal of Molecular Sciences·Liangfu JiangXiaofeng Jia
Feb 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chengbin XueXiaosong Gu
Mar 20, 2021·Signal Transduction and Targeted Therapy·Kieran JoyceAbhay Pandit
Apr 4, 2021·Pharmaceutics·Mahdi YonesiDaniel González-Nieto
May 18, 2021·Bioactive Materials·Wen XueBin Duan

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