Application of microdialysis to evaluate the efflux transport of estradiol 17-beta glucuronide across the rat blood-retinal barrier

Journal of Neuroscience Methods
Kazunori KatayamaKen-ichi Hosoya

Abstract

The purpose of the present study was to evaluate vitreous humor/retina-to-blood efflux transport in rats and determine the efflux transport of estradiol 17-beta glucuronide (E17betaG) across the blood-retinal barrier (BRB) by the use of microdialysis. [(3)H]E17betaG and [(14)C]D-mannitol, which were used as a model compound for amphipathic organic anions and a bulk flow marker, respectively, were injected into the vitreous humor of rat eye, and a microdialysis probe was placed in the vitreous humor. [(3)H]E17betaG and [(14)C]D-mannitol were bi-exponentially eliminated from the vitreous humor after vitreous bolus injection. The elimination rate constant of [(3)H]E17betaG during the terminal phase was 1.9-fold greater than that of [(14)C]D-mannitol and reduced the level of [(14)C]D-mannitol in the retinal presence of 0.3 mM E17betaG, suggesting that [(3)H]E17betaG is transported via a carrier-mediated efflux transport process across the BRB. The efflux transport of [(3)H]E17betaG was significantly inhibited by organic anions, such as probenecid, sulfobromophthalein, digoxin, and dehydroepiandrosterone sulfate, whereas it was not inhibited by p-aminohippuric acid. In conclusion, the efflux transport of [(3)H]E17betaG across the ra...Continue Reading

References

Jul 1, 1992·Journal of Neurochemistry·P Louzada-JúniorJ Coutinho-Netto
Jan 1, 1983·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·J Horowitz, M Sears
Nov 1, 1981·Journal of Pharmacobio-dynamics·K YamaokaT Uno
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·B NoéP J Meier
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B GaoP J Meier
Aug 23, 2000·Pflügers Archiv : European journal of physiology·T SekineH Endou
Jan 10, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J Y LiW M Pardridge
Feb 22, 2001·Experimental Eye Research·S Macha, A K Mitra
Jan 1, 2003·Biochimica Et Biophysica Acta·B Hagenbuch, P J Meier
Jan 6, 2004·Current Eye Research·Sridhar DuvvuriAshim K Mitra
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tsuyoshi MikkaichiTakaaki Abe
Mar 24, 2004·Investigative Ophthalmology & Visual Science·Ken-ichi HosoyaMasatoshi Tomi
Oct 23, 2004·Pharmaceutical Research·Karin TunbladE Niclas Jonsson

❮ Previous
Next ❯

Citations

Jul 2, 2009·Pharmaceutical Research·Ken-ichi Hosoya, Masanori Tachikawa
May 5, 2010·Expert Opinion on Drug Metabolism & Toxicology·Masatoshi Tomi, Ken-ichi Hosoya
Jan 16, 2010·Experimental Eye Research·Daisuke YoneyamaKen-Ichi Hosoya
May 3, 2018·Expert Opinion on Drug Metabolism & Toxicology·Yoshiyuki KuboKen-Ichi Hosoya
Oct 4, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shinobu FujiiKen-Ichi Hosoya
Mar 14, 2018·Pharmaceutical Research·Yoshiyuki KuboKen-Ichi Hosoya
Oct 3, 2013·Fluids and Barriers of the CNS·Shin-Ichi AkanumaKen-Ichi Hosoya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.