Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage

Microbial Biotechnology
Kate Le CocqDavid R Davies

Abstract

Proliferation of filamentous fungi following ingress of oxygen to silage is an important cause of dry matter losses, resulting in significant waste. In addition, the production of mycotoxins by some filamentous fungi poses a risk to animal health through mycotoxicosis. Quantitative assessment of fungal growth in silage, through measurement of ergosterol content, colony-forming units or temperature increase is limiting in representing fungal growth dynamics during aerobic spoilage due to being deficient in either representing fungal biomass or being able to identify specific genera. Here, we conducted a controlled environment aerobic exposure experiment to test the efficacy of a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) to detect the proliferation of fungal biomass in six silage samples. We compared this to temperature which has been traditionally deployed in such experiments and on-farm to detect aerobic deterioration. In addition, we quantified ergosterol, a second marker of fungal biomass. After 8 days post-aerobic exposure, the ergosterol and ELISA methods indicated an increase in fungal biomass in one of the samples with a temperature increase observed after 16 days. A comparison of the methods wit...Continue Reading

References

Jun 21, 1991·Science·T A Waldmann
Feb 1, 1990·The Journal of Applied Bacteriology·M K Woolford
May 25, 2004·Methods in Molecular Biology·Dante Javier BuenoGuillermo Oliver
Sep 17, 2004·Journal of Microbiological Methods·Cecilia Mille-LindblomLars J Tranvik
Sep 7, 2007·International Journal of Food Microbiology·Ludwig Niessen
Oct 24, 2007·Journal of Applied Microbiology·F Rossi, F Dellaglio
Feb 21, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Johanna Fink-Gremmels
May 9, 2008·Clinical and Vaccine Immunology : CVI·Christopher R Thornton
Dec 3, 2008·Journal of Microbiological Methods·Mogens NicolaisenAnnemarie Fejer Justesen
Apr 5, 2012·Journal of Visualized Experiments : JoVE·Christopher ThorntonSamir Agrawal
Jan 8, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Fei ZhaoXiaoqin Ye
Feb 23, 2013·Veterinary Microbiology·Claudia CafarchiaDomenico Otranto
Mar 1, 2013·Journal of Applied Microbiology·V A AlonsoL R Cavaglieri
Oct 13, 2014·Journal of Dairy Science·M D MarchD J Roberts
Jan 21, 2016·Journal of Applied Microbiology·B F CarvalhoR F Schwan
Mar 15, 2018·Medical Mycology·Seyedmojtaba SeyedmousaviJacques Guillot
Apr 25, 2018·Journal of Dairy Science·W K Coblentz, M S Akins

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
infrared spectroscopy

Software Mentioned

Genstat

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.