Application of multiplex arrays for cytokine and chemokine profiling of bile

Cytokine
Troy KempJill Koshiol

Abstract

Gallbladder disease is highly related to inflammation, but the inflammatory processes are not well understood. Bile provides a direct substrate in assessing the local inflammatory response that develops in the gallbladder. To assess the reproducibility of measuring inflammatory markers in bile, we designed a methods study of 69 multiplexed immune-related markers measured in bile obtained from gallstone patients. To evaluate assay performance, a total of 18 bile samples were tested twice within the same plate for each analyte, and the 18 bile samples were tested on two different days for each analyte. We used the following performance parameters: detectability, coefficient of variation (CV), intraclass correlation coefficient (ICC), and percent agreement (concordance among replicate measures above and below detection limit). Furthermore, we examined the association of analyte levels with gallstone characteristics such as type, numbers, and size. All but 3 analytes (Stem Cell Factor, SCF; Thrombopoietin, TPO; sIL-1RI) were detectable in bile. 52 of 69 (75.4%) analytes had detectable levels for at least 50% of the subjects tested. The within-plate CVs were ⩽25% for 53 of 66 (80.3%) detectable analytes, and across-plate CVs were ⩽2...Continue Reading

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Citations

Mar 17, 2016·Biochimica Et Biophysica Acta·Jaime A EspinozaJuan C Roa
Apr 20, 2018·Journal of Gastroenterology and Hepatology·Zhiwei LiuJill Koshiol
Jan 29, 2019·Environmental Science and Pollution Research International·Martyna Pajewska-SzmytRenata Gadzała-Kopciuch
Jun 12, 2021·Advanced Science·Chao LiuGuozhen Liu

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