Application of near-infrared reflectance spectroscopy (NIRS) to the evaluation of carotenoids content in maize

Journal of Agricultural and Food Chemistry
Oreste V Brenna, Nicola Berardo

Abstract

The importance of including antioxidant compounds in the diet is well recognized. These compounds remediate the detrimental activity on animal cells of the so-called reactive oxygen substances (ROS). Many papers have reported on the determination of both hydrophilic and hydrophobic antioxidant compounds present in a large number of vegetables, and all methods involve the extraction from the matrix of the compounds to be determined. Because some problems may arise, such as the completeness of the extraction and the stability of the extracted compound during the extraction steps, the possibility of analyzing these compounds in the native matrix would be useful. Here is reported the application of near-infrared spectroscopy (NIRS) to the determination of the content of carotenoids in maize, comparing the obtained data with those derived from high-performance liquid chromatography (HPLC) determination of the extract obtained from the same samples. Equations for predicting carotenoid content in maize were derived using scores from modified partial least-squares (MPLS) as independent variables. Cross-validation procedures indicated good correlations between HPLC values and NIRS estimates. The results show that NIRS, a well-establishe...Continue Reading

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Citations

Nov 30, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C J PozniakJ M Clarke
Mar 28, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xuecai ZhangJiankang Wang
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Jun 3, 2005·Phytochemical Analysis : PCA
Aug 25, 2007·Journal of Agricultural and Food Chemistry·Haifeng YangYuchang Qin

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