PMID: 3748819Aug 26, 1986Paper

Application of new catalytic phosphate protecting groups for the highly efficient phosphotriester oligonucleotide synthesis

Nucleic Acids Research
V A Efimov Ovchinnikov YuA

Abstract

An effective procedure for the synthesis of oligonucleotides by the phosphotriester method has been developed. The procedure is based on the use of phosphate protecting groups enabling O-nucleophilic intramolecular catalysis in the reaction of internucleotide bond formation under the action of arylsulfonyl chlorides and their derivatives. Using this new procedure, the time needed to perform one elongation step on polymer support is 7-8 min. The effectiveness of the methodology has been demonstrated in the synthesis of many oligodeoxyribonucleotides of different length with high yields.

Citations

Jan 17, 2014·Journal of Chemical Biology·James I MurrayRudiger Woscholski
Jan 1, 1999·Bioorganic & Medicinal Chemistry Letters·V A EfimovO G Chakhmakhcheva
Sep 6, 2011·Nucleosides, Nucleotides & Nucleic Acids·Vladimir A EfimovOksana G Chakhmakhcheva
Sep 6, 2011·Nucleosides, Nucleotides & Nucleic Acids·Oksana Chakhmakhcheva
Dec 7, 2007·Nucleosides, Nucleotides & Nucleic Acids·Vladimir A EfimovOksana G Chakhmakhcheva
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·V A EfimovO G Chakhmakhcheva
Jun 20, 2009·Bioorganicheskaia khimiia·V A EfimovO G Chakhmakhcheva
Nov 11, 2010·Bioorganicheskaia khimiia·V A EfimovO G Chakhmakhcheva
Aug 7, 2017·Beilstein Journal of Organic Chemistry·Harri Lönnberg

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