Application of novel electrospun nanofibrous membranes with different modifications for immobilising glutamine synthetase

Journal of the Science of Food and Agriculture
Bo LiuPing Li

Abstract

Theanine synthesis is of interest because of the physiological and pharmacological benefits of theanine. Glutamine synthetase (GS, EC 6.3.1.2) from Pseudomonas taetrolens can be used for theanine biosynthesis. In this study, GS was immobilised in polyvinyl alcohol electrospun nanofibres, and the properties of immobilised GS with different solidifying modifications were studied. Electrospinning is a good method for enzyme immobilisation. When carbon nanotubes were used as an adsorbent, the activity retention of immobilised GS was 92.3%. With glutaraldehyde as a crosslinker, the enzyme activity retention was only 50.7%; however, by adding collagen or peptide to the electrospinning solution with glutaraldehyde, the activity retention could be improved to 85.9 or 59.6% respectively. Scanning electron micrographs showed that the modifiers induced morphological changes in the nanofibres. The optimum pH and temperature of immobilised GS also changed. In addition, the reusability and storage stability of immobilised GS with glutaraldehyde were much better than those of immobilised GS with carbon nanotubes. A novel method has been established in this study for the immobilisation of GS in electrospun nanofibrous membranes. Carbon nanotub...Continue Reading

References

Jun 5, 1998·Science·J LiuR E Smalley
Nov 7, 2003·Applied and Environmental Microbiology·Hideyuki SuzukiHidehiko Kumagai
Mar 18, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hongliang JiangKangjie Zhu
Aug 26, 2006·Biological Psychology·Kenta KimuraHideki Ohira
Jan 1, 1992·Bioscience, Biotechnology, and Biochemistry·T KimuraY Tonomura

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