Abstract
The continuous monitoring of a process based on the culture of Sf9 insect cells and infection by a baculovirus as a vector to obtain recombinant VP2 protein is studied. On-line OUR determination is based on the direct oxygen measurement in the cell culture vessel and the application of the dynamic method. This approximation allows a proper description of cell growth, with precise identification of the balanced cell growth end and the most important action times in the process, as virus infection time and final cell harvesting. A detailed study of the OUR profiles allows on-line monitoring of the effects of infection and expression protein process, a tool enabling the automatisation of the protein production process in a baculovirus-insect cell system. These parameters have been defined as time of action (TOAs), and include the most relevant actions to take in these type of processes: time of infection (TOI), time of feeding (TOF) and time of harvesting (TOH).
References
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K VuoriK I Kivirikko
Jul 1, 1990·Journal of Biotechnology·B SchopfJ E Bailey
Sep 1, 1992·Biotechnology Progress·T J WickhamH A Wood
Oct 1, 1992·Enzyme and Microbial Technology·R I ScottC H Ferguson
Feb 15, 1991·Gene·D C TessierT Vernet
Feb 1, 1993·Applied Microbiology and Biotechnology·S ReuvenyJ Shiloach
Jan 1, 1994·Cytotechnology·T K WongS Reid
Nov 14, 1997·Current Opinion in Biotechnology·R D Possee
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y C LeeF Murad
Oct 12, 2000·Biotechnology Progress·A ZeiserA A Kamen
Jun 29, 2002·Journal of Clinical Microbiology·Minako IkomaSytske Welling-Wester
May 2, 2003·Journal of Biomedicine & Biotechnology·Lindsay J. StanbridgeNorman J. Maitland
Nov 13, 2008·Cytotechnology·Volker M GorenfloJames M Piret
Jan 1, 1996·Cytotechnology·V Jäger
Jan 1, 1996·Cytotechnology·L A Palomares, O T Ramirez
Citations
Oct 17, 2008·Applied Microbiology and Biotechnology·Nuno CarinhasPaula M Alves
Nov 5, 2013·Journal of Bioscience and Bioengineering·Leticia Liste-CallejaJordi Joan Cairó
Feb 10, 2016·Molecular Therapy. Methods & Clinical Development·Timothy Weixin KwangShu Wang
May 22, 2009·Biotechnology and Bioengineering·Vicente BernalPaula M Alves
Jun 8, 2011·Biotechnology Journal·Marilena Martins PamboukianAldo Tonso
Nov 29, 2011·Journal of Biotechnology·J GálvezF Gòdia
Jul 17, 2019·Applied Microbiology and Biotechnology·I Martínez-MongeJ J Cairó
Oct 6, 2018·Applied Microbiology and Biotechnology·I Martínez-MongeJ J Cairó
Sep 14, 2021·Frontiers in Bioengineering and Biotechnology·Nina IhlingJochen Büchs