Application of optical isomer analysis by diastereomer derivatization GC/MS to determine the condition of patients with short bowel syndrome

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yoshito InoueTomiko Kuhara

Abstract

To establish a method for separating the optical isomers of lactic acid, we modified the derivatization steps in our procedure for urinary mass-screening for inborn errors of metabolism. For chiral recognition, we chose O-trifluoroacetyl-(-)-menthylation derivatization instead of our previous method, trimethylsilyl derivatization, and the samples were then analyzed under GC/MS by capillary gas chromatography on a DB-5MS column. This method can be used to follow-up the condition of a patient with short bowel syndrome and to prevent onset and/or seizure. d-Lactic acid was also isolated from the urine of healthy controls as one of the main peaks in the chromatogram.

References

Jun 9, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·O O OmoleG A Zello
Jul 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·E Haschke-BecherC Bachmann
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Julia B EwaschukDion R Brocks
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Julia B EwaschukGordon A Zello

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Citations

Aug 25, 2011·Scandinavian Journal of Clinical and Laboratory Investigation·Casper NielsenErland J Erlandsen
Aug 13, 2015·Journal of Pharmaceutical and Biomedical Analysis·Chien-Ming ChenHan-Yin Yu
Jun 5, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yukina MatsuiKenji Kano
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Mar 3, 2009·Journal of Pharmaceutical and Biomedical Analysis·Elliott J FrancoOliver Hofstetter

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