Application of over-run thin-layer and gas-liquid chromatography in the separation of closely related C19O2 steroids

Journal of Chromatography
B P Lisboa, U Hoffmann

Abstract

An improved method for the separation of epimeric C19O2 steroids and their related allylic alcohols is described. In this method, the steroids are first separated by over-run thin-layer chromatography, and the unresolved groups are further analysed as free or as trimethylsilyl ether derivatives by gas-liquid chromatography. The behaviour of twenty-one C19O2 steroids was investigated by thin-layer chromatography in four systems and by gas-liquid chromatography in four liquid phases. All steroid pairs of similar polarity were resolved by the combination of these two fractionation procedures.

References

Sep 1, 1966·The Journal of Clinical Endocrinology and Metabolism·H L BradlowT F Gallagher
Nov 1, 1963·The Journal of Endocrinology·H BREUERJ K NORYMBERSKI

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Citations

Nov 1, 1982·Journal of Steroid Biochemistry·C D Kochakian
Nov 1, 1975·European Journal of Biochemistry·U HoffmannB P Lisboa
Jan 1, 1986·Avian Pathology : Journal of the W.V.P.A·R L WitterA M Fadly
Sep 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·S C BurgessT F Davison
Jan 1, 1977·Avian Pathology : Journal of the W.V.P.A·O R Kaaden
Jul 1, 1979·Avian Pathology : Journal of the W.V.P.A·V V Bulow, D O Schmid
Apr 1, 1981·Avian Pathology : Journal of the W.V.P.A·K A Schat
Apr 1, 1984·Avian Pathology : Journal of the W.V.P.A·P C Powell, M Rennie

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