Application of Pb(II) to probe the physiological responses of fungal intracellular vesicles

Ecotoxicology and Environmental Safety
Zhongquan JiangZhen Li

Abstract

Vesicles (Ves) within fungal cells are the critical linkage between intracellular and extracellular systems. This study explored the application of Pb2+ to probe the physiology of intracellular Ves in Rhodotorula mucilaginosa (Rho). At low Pb2+ levels (0-500 mg/L), there was no evident change in the content of extracellular polymeric substances (EPS) or microbial activity. At medium-high levels (1000-2000 mg/L), the sizes of Ves within the Rho cells were significantly enlarged, with abundant lead nano-particles (Pb NPs) formed either on the cell surface or interior, whereas the EPS content and bioactivity were still stable. At a high level (2500 mg/L), the Rho cells were severely deformed, with cell counts reduced by more than 99%. However, the EPS contents and the respiration rate of the surviving cells dramatically increased to the maximum values (i.e., 1785 mg/1010 cells and 37 mg C 10-10 cells h-1, respectively). The Ves surface adsorbed Pb cations with higher density, compared with the cell membrane. Moreover, fusion of some Ves to the membrane (functioning in transport) was observed under transmission electron microscope (TEM). Three pathways of detoxification via intracellular Ves were finally proposed, i.e., Ve-mediated...Continue Reading

References

Jan 1, 1978·Annals of the New York Academy of Sciences·G Poste, D Papahadjopoulos
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Novick, R Schekman
Apr 1, 1977·Life Sciences·J H Fendler, A Romero
Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
Jan 1, 1983·Current Topics in Cellular Regulation·H G Williams-Ashman
Aug 1, 1995·Current Opinion in Cell Biology·M K Bennett
Feb 1, 1995·Journal of Industrial Microbiology·H TohoyamaT Murayama
Mar 15, 1996·Science·R Schekman, L Orci
Jan 1, 1996·Annual Review of Microbiology·S Silver, L T Phung
Sep 4, 1997·Nature·J F PresleyJ Lippincott-Schwartz
Feb 4, 1999·Nature Medicine·A GuptaS Silver
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KlausC G Granqvist
Feb 29, 2000·Journal of Bacteriology·M García-DomínguezJ C Reyes
Oct 14, 2000·Archives of Environmental Contamination and Toxicology·S M Macfie, P M Welbourn
May 4, 2001·Biochimica Et Biophysica Acta·R B CampbellR M Straubinger
Aug 10, 2002·Science·Dennis E Discher, Adi Eisenberg
Dec 31, 2002·Current Opinion in Structural Biology·Philip R Evans, David J Owen
Sep 25, 2003·Bioprocess and Biosystems Engineering·Dae Haeng Cho, Eui Yong Kim
Oct 24, 2003·Antonie van Leeuwenhoek·Paul Illmer, Christian Erlebach
Oct 23, 2004·International Journal of Cancer. Journal International Du Cancer·Anna Janowska-WieczorekMariusz Z Ratajczak
Nov 6, 2004·Applied and Environmental Microbiology·Elian-Simplice YaganzaRussell J Tweddell
Mar 21, 2009·Trends in Microbiology·Arturo CasadevallMarcio L Rodrigues
May 29, 2009·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·J-L QuY-P Liu
Sep 5, 2009·Microbiology·Helene C EisenmanArturo Casadevall
Oct 2, 2009·Neoplasia : an International Journal for Oncology Research·Thorsten JungMargot Zöller
Dec 19, 2009·Microbiology·Geoffrey Michael Gadd
Apr 9, 2010·FEMS Microbiology Reviews·Robert Wysocki, Markus J Tamás
Apr 27, 2010·Current Opinion in Cell Biology·Frederick M Hughson, Karin M Reinisch
Mar 23, 2011·Nature Biotechnology·Lydia Alvarez-ErvitiMatthew J A Wood
Jun 10, 2011·Journal of Translational Medicine·Sascha KellerPeter Altevogt
Jan 21, 2012·Bioresource Technology·Rajdeep Chakravarty, Pataki C Banerjee
May 19, 2012·Inorganic Chemistry·Vicky Mah, Farideh Jalilehvand
May 23, 2012·Radiation Research·Ammar H J Al-MayahMunira A Kadhim
Dec 20, 2012·Nature Communications·Ke TangBo Huang
Apr 18, 2013·Current Microbiology·Rita R PerezEduardo V Soares
Aug 22, 2014·Journal of Extracellular Vesicles·Laura Ann MulcahyDavid Raul Francisco Carter
Feb 1, 2018·Chemical Reviews·Huilin ShaoHakho Lee

❮ Previous
Next ❯

Citations

Oct 13, 2020·Applied Microbiology and Biotechnology·Mengxiao WangZhen Li
Apr 27, 2021·Environmental Science and Pollution Research International·Bing ShanJunman Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Deutsche medizinische Wochenschrift
T MeinertzT Pop
The Journal of Surgical Research
Mokenge P Malafa, Leslie T Neitzel
The Journal of Surgical Research
Kevin T BarnettMokenge P Malafa
Ai zheng = Aizheng = Chinese journal of cancer
Yong-Heng DongXin-Bin Gu
© 2021 Meta ULC. All rights reserved