Application of plant DNA markers in forensic botany: genetic comparison of Quercus evidence leaves to crime scene trees using microsatellites

Forensic Science International : Synergy
Kathleen J CraftMary V Ashley

Abstract

As highly polymorphic DNA markers become increasingly available for a wide range of plant and animal species, there will be increasing opportunities for applications to forensic investigations. To date, however, relatively few studies have reported using DNA profiles of non-human species to place suspects at or near crime scenes. Here we describe an investigation of a double homicide of a female and her near-term fetus. Leaf material taken from a suspect's vehicle was identified to be that of sand live oak, Quercus geminata, the same tree species that occurred near a shallow grave where the victims were found. Quercus-specific DNA microsatellites were used to genotype both dried and fresh material from trees located near the burial site and from the material taken from the suspect's car. Samples from the local population of Q. geminata were also collected and genotyped in order to demonstrate that genetic variation at four microsatellite loci was sufficient to assign leaves to an individual tree with high statistical certainty. The cumulative average probability of identity for these four loci was 2.06x10(-6). DNA was successfully obtained from the dried leaf material although PCR amplification was more difficult than amplifica...Continue Reading

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Citations

Jun 9, 2009·International Journal of Legal Medicine·Gianmarco FerriGiovanni Beduschi
Aug 13, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hitomi S KikkawaShinichi Suzuki
Jan 9, 2010·Forensic Science International : Synergy·Marcel C M EurlingsBarbara Gravendeel
Mar 11, 2010·Forensic Science International. Genetics·Jin-Wen ChenYing Liu
Mar 24, 2009·Journal of Forensic Sciences·Christopher HowardRod Peakall
Jun 8, 2011·Journal of Forensic Sciences·Lindsay AllgeierHeather Miller Coyle
Aug 10, 2013·Medicine, Science, and the Law·Arati Iyengar, Sibte Hadi
Dec 2, 2017·Journal of Forensic Sciences·Tushar SrivastavaNathan H Lents
Sep 22, 2017·PLoS Genetics·Miguel ArenasAntonio Amorim
Apr 9, 2021·Forensic Science, Medicine, and Pathology·Sarah IshakJennifer M Young
Apr 30, 2020·Saudi Journal of Biological Sciences·Saqer S AlotaibiReem Alhamed

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